不同水质水对体外溶血试验的影响

Experimental study on hemocytolysis test of various character water

目的通过比较饮用水、去离子水、纯水、双蒸水、纯净水及灭菌注射用水等不同水质水在体外溶血试验中的溶血度,为体外溶血试验提供较好的阳性对照。方法采用常规体外试管法(肉眼观察法)和改进的体外溶血性试验法(分光光度法)测定不同水质水对兔血红细胞的溶血度。结果这几种水质水在体外均有溶血作用,灭菌注射用水溶血程度最大,饮用水溶血程度最小,其它几种水质溶血程度差异不大,肉眼观察法和分光光度法结果一致。结论在本试验条件下,几种水质水对兔血红细胞的溶血度为灭菌注射用水>纯净水>双蒸水>纯水>去离子水>饮用水,溶血试验阳性对照推荐使用灭菌注射用水。

Objective Through comparing the drinking water, deionized water, pure water, double steamed water, purified water and sterilized water for injection and so on the various character water in vitro hemolysis test of hemolysis degree, we hope to provide the better positive control. Methods The hemocytolysis of various character water was observed by means of the routine test tube method in vivo （ macroscopic observation methods） and modified hemolytic test method in vitro （spectrophotometry）. Results Hemolytic crisis occur between the six character water, the hemolysis degree of sterilized water for injection is the largest, drinking water is minimum, the difference of several other character water is small, macroscopic observation method and spectrophotometry results are consistent. Conclusions In this test conditions, several character water to the rabbit red blood cells of hemolysis degree is sterilization water for injection 〉 purified water 〉 double steamed water 〉 pure water 〉 deionized water 〉 drinking water, we recommended sterilized water for injection for hemolysis test positive control.