小鼠肺纤维化模型中衰老对肺干细胞的影响

Impact of aging on repair capability of lung stem cells in a mouse model of pulmonary fibrosis

目的研究衰老对肺干细胞在博来霉素致肺纤维化小鼠模型中修复能力的影响。方法利用博来霉素处理年轻,年老小鼠建立肺纤维化模型,通过流式细胞术等方法比较肺组织干细胞在年轻、年老小鼠肺纤维化模型中的比例及增殖情况,研究衰老对肺组织干细胞的损伤修复能力的影响。结果在博来霉素致肺纤维化的模型中,年老小鼠相对年轻小鼠肺组织干细胞增殖明显降低,肺上皮干细胞和肺间质干细胞比例显著下降。结论在衰老过程中肺组织干细胞在肺纤维化损伤后修复能力明显减弱。

Objective To study the impact of aging on the capability of regeneration and differentiation of lung stem cells in mice with bleomycin-induced pulmonary fibrosis. Methods The proportion of lung stem cells in mice after bleomycin treatment was analyzed by flow cytometry. The repair capability of lung stem cells in young and old mice was examined by immunohistochemistry and flow cytometry. Results The ratio of lung epithelial stem cells/lung total cells and lung mesenchymal stem cells/lung total cells were significantly decreased in the aged mice. The proliferating lung stem cells/lung total cells reduced in old mice during the repair process of bleomycin-induced pulmonary fibrosis. Conclusion The repair capability of lung stem cells during bleomycin-induced pulmonary fibrosis is decreased in aging.