动物模型评价化妆品原料美白功效的研究

Whitening efficacy assessment for cosmetic materials in animal model

目的建立化妆品原料美白功效评价动物模型并应用。方法紫外线连续照射7 d造成花色豚鼠皮肤黑化模型,在褐色无毛部位连续涂抹样品30 d。Maxmeter仪检测皮肤黑色素指数(MI)和红色素指数(EI)的变化。安乐处死动物后取皮肤组织,多聚甲醛固定、石蜡切片,多巴胺染色和氨银染色,对组织切片进行图像分析。结果 UV照射豚鼠皮肤后,MI指数升高,组织学观察显示黑色素颗粒增大和表皮层分布增多。经白藜芦醇-熊果苷复配美白原料测试,皮肤颜色MI指数显著下降,EI指数升高。组织学显示呈多巴染色阳性的黑素颗粒明显抑制,氨银染色区域在表皮内的分布下降。结论利用紫外线造成实验动物皮肤黑化模型,运用皮肤生物物理学检测和组织学染色分析技术可以用于美白化妆品原料的功效评价和机制研究。

Objective To establish the assessment method for whitening efficacy of cosmetic materials by animal model and applications. Methods Guinea pigs were irradiate with UVB for 7 days to induce skin darkening model thereafter to assess the whitening efficacy of cosmetic materials at brown part through treatment 30 days continuously. The levels of melanin index（MI） and erythemin index（EI） were analysed by Maxmeter MX18. The skin tissues were stripped, paraformaldehyde fixed and paraffin sections following the animal euthanasia. The morphological observed and image analysis were detected following Dopamine or Fontana -Masson histochemieal staining. Results The MI index of skin and melanin granular in the section of guinea pig were increased significantly after UVB radiation. Following the resveratrol-- arbutin blend treatment, it was showed that MI index were decreased, the melanin granular significantly inhibit by dopamine positive staining and Fontan-Masson positive staining distribution in the epidermal were decreased obviously. Conclusions The skin biophysics detecting combine histochemical staining and image analysis through UV induced skin darkening animal model, which could be used for the assessment of whitening efficacy of cosmetic materials and mechanism research of melanogenesis.