紫芝不亲和性因子分析

Incompatibility factors of Ganoderma sinense

为开展紫芝的杂交育种,对6个紫芝菌株的不亲和性因子进行分析。通过原生质体单核化,6个紫芝获得9个不同亲本型的单核化菌株,共测得7个不同的A因子和7个不同的B因子,A、B因子的多态性各占单核体的77.8%,表明紫芝有较丰富的交配型因子,为紫芝的杂交育种奠定基础。

In order to carry out the cross-breeding of Ganoderma sinense, mating type factors in six strains of G. sinense were analyzed. Using the protoplast monokaryogenesis technique, nine monocaryons of different parental type were derived from the six strains. There were seven different A factors and seven different B factors in the nine monokaryons, respectively accounting for 77.8% of the monocaryons. It suggested that there was a wealth of mating type factor in G. sinense, which could lay the foundation for the cross-breeding of G. sinense.