摘要
目的探讨Aβ1-42寡聚体对小胶质细胞白细胞介素（IL）- 1β和肿瘤坏死因子（TNF）-α表达的影响，以及海风藤提取物的干预作用。方法将小鼠源小胶质细胞株BV2随机分为3组：空白对照组、Aβ1-24组（给予Aβ1-42寡聚体）、Aβ1-42＋海风藤提取物组（在给予Aβ1-42寡聚体的基础上加用海风藤提取物干预）。按实验要求作用48h后，实时荧光定量聚合酶链反应（RT-PCR）方法检测各组细胞IL-Iβ、TNF-amRNA含量，蛋白印迹法（Western blot）检测各组细胞IL-1β、TNF-α蛋白表达量。结果Aβ1-42寡聚体组IL-1β、TNF-dmRNA表达量分别为对照组的（21．9±0．4）倍、（3．8±0．3）倍，较对照组显著升高（t=88．67、16．47，均P=0．000）；Aβ1-42寡聚体＋海风藤提取物组IL-1β、TNF-amRNA表达量分别为对照组的（8．3±0．3）倍、（1．3±0．2）倍，但较Aβ1-42寡聚体组明显下降（t=57．70、14．71，均P=0．000）。Aβ1-42寡聚体组IL-1β、TNF-α蛋白表达量分别为83．3±8．5、32．0±3．6，较空白对照组25．2士13．9、2．6±1．7明显增加（t=7．46、11．74，均P=0．000）；Aβ1-42寡聚体＋海风藤提取物组IL-1β、TNF-α蛋白表达量分别为59．3±2．7、21．7±4．5，较Aβ1-42寡聚体组明显减少（t=3．08、4．38，P=0．022、0．005）。结论海风藤提取物可以明显抑制Aβ1-42寡聚体激活小胶质细胞引起的IL-1β、TNF-amRNA及蛋白的过量表达。
Objective To investigate the effect of oligomeric Aβ142 on the expressions of interleukin （IL）-1β and tumor necrosis factor（TNF）-α in microglia cells, and to observe the impact of piper kadsura ohwi extracts induced by oligomeric Aβ1-42. Methods Murine microglial cell line BV2 were randomly divided into 3 groups： control group （without any intervention）, oligomericAAβ1-42 group （intervened by oligomeric Aβ1-42 ）, oligomeric Aβ1-42 piper kadsura ohwi extracts group （intervened by oligomeric Aβ1-42 and piper kadsura ohwi extracts）. After 48 hours of the intervention, levels of IL-1β and TNF-α were determined by RT-PCR and Western blotting. Results Compared with the control group, the levels of IL-1β and TNF-a mRNA were significantly increased by （21.9± 0.4） folds and （3.84±0.3） folds in oligomeric Aβ1-42 group （t=88.67, 16.47, both P〈0. 001） and by （8. 3_--＋-0.3） folds and （1.3-4-0.2） folds in oligomeric Aβ1-42 ＋ Piper kadsura ohwi extracts group （t= 30. 97, P〈0. 001; t=l. 77, P〈0. 05）. The Aβ1-42induced expressions of IL-1β and TNF-α mRNA were markedly decreased in oligomeric Aβ1-42 Piper kadsuraohwi extracts group （t= 57.70, 14.71, both P〈0. 001）. Western blotting showed that the protein expressions of IL-lβ and TNF-α were increased in oligomeric Aβ1-42 group as compared with the control group [（83. 3±8. 5） vs（25.2± 13.9）, （32. 0±3. 6）vs（2.6±1.7）, t=7.46, 11.74, both P〈0.001D. The protein express ions of IL-β3 and TNF-α were decreased in oligomericAβ1-42 ＋ Piper kadsura ohwi extracts group as comparedwith the oligomeric Aβ1-42 group[（59.3±2.7） w （83.3±8.5）, （21.7±4.5） vs （32.0±3.6）, t= 3.08, 4.38, P〈0.05 or 0.01]. Conclusions Piper kadsura ohwi extracts can inhibit the microglia over-expressions of IL-1β and TNF-αactivated by oligomeric Aβ1-42
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2014年第3期311-314,共4页
Chinese Journal of Geriatrics
基金
国家自然基金计划项目（30973816）
山东省科技攻关计划项目（2009HDO15）
