甲醛对鼠BM-MSCs细胞系的遗传毒性影响

Genotoxic effect of formaldehyde on mouse BM-MSCs

目的研究甲醛对BM—MSCs的遗传毒性，从而为甲醛导致白血病的发作提供生物学依据。方法采用噻唑篮比色（Mrrr）法检测BM—MSCs的增殖活性；用彗星、KCI—SDS沉淀、姐妹染色单体互换（SCE）和微核（MN）柃测甲醛对BM—MSCs的遗传毒忡效应。结果75～200μmol／L。甲醛可呈剂量依赖抑制BM—MSCs的增殖（P〈0．01），诱导BM—MSCsDNA断裂效应呈现先增高后降低趋势，在125μmol/L时，达到峰值，其中75～150μmol／L甲醛作用较对照组显著升高（P〈0．01）；甲醛在≥125μmol／L，时可以明显引起BM—MSCsDNA-蛋白交联（DPCs）、SCE和MN的形成，较对照组显著升高（P〈0．01）。结论甲醛可抑制BM—MSCs的增殖活性，并且对BM—MSCs具有一定的遗传毒性效应。

Objective To find potential genotoxic effect of formaldehyde on BM-MSCs, for providing the biological evidence for the formaldehyde to lead to leukemia. Methods Proliferation activity was measured by MTT assay; Genotoxic effect was detected by comet assay, KC1-SDS precipitation assay, sister chromatid exchange （SCE） test and micronucleus （MN） test. Results 75 - 200 μmol/L formaldehyde can inhibit the BM-MSCs proliferation in a dose-dependent manner （P 〈0. 01 ） ; DNA strand breakage increased gradually at increasing concentrations below 125 μmol/L, however, when formaldehyde concentration was over 125 μmol/L, DNA strand breakage decreased. There were significant rises in 75 - 150 μmoL/L formaldehyde groups compared with control group （P 〈 0. O1 ） ; In- duction of DNA-protein crosslinks （ DPCs）, SCE and MN in /〉 125 μmol/L formaldehyde groups were significant higher than in control group （P 〈 0. 01 ）. Conclusions Formaldehyde can inhibite the BM-MSCs proliferation ac- tivity, and have a genotoxic effect on the BM-MSCs.