大鼠膝关节周围微小动脉网分布及计数法的评价

Evaluation methods for small vascular network distribution and counting around the knee joint in rats

背景:随着骨组织工程研究的不断深入和对骨代谢过程的不断认识,分析组织工程骨的血液供应和营养状况已成为目前的研究重点。目的:比较和评价常用的微小血管检测方法,明确各种方法的利弊,为研究组织工程骨内微血管的分布和计数提供借鉴意义。方法:分别用3种常用的血管评价法对SD大鼠膝关节周围微小动脉网进行分析对比,18只SD大鼠均分为免疫组化组、血管造影组、CT扫描重建组。结果与结论:免疫组化技术能观察到血管的分布范围,但是无法评价血管空间分布的整体情况。血管造影技术和CT检查则可以提供形象的血管空间分布图,但是CT检测时部分微小血管显示不清。免疫组化检出(26.50±3.02)条血管数明显多于血管造影(14.12±1.47)条和CT检测(9.00±1.79)条,其中CT检测组可检出的血管数最少。说明免疫组化和血管造影可以为微小血管提供微观和宏观的评价方法,从两个层面一起观察,更好的分析血管的情况,对微血管的评价有很好的借鉴意义。

BACKGROUND： With the deepening of bone tissue engineering research and bone metabolism understanding, it is a hotspot to analyze the blood supply and nutritional status of tissue-engineered bone. OBJECTNE： To compare different methods for evaluating small vascular network distribution around the knee joint in rats in order to provide a guideline for the study of microvascular network in tissue-engineered bone. METHODS： Eighteen Sprague-Dawley rats were randomly divided into three groups, with six rats in each group. Three commonly methods were used to evaluate the small vascular network around the knee joint in rats： immunohistochemistry analysis, angiography analysis, and CT scans and reconstruction analysis. RESULTS AND CONCLUSION： The microstructure of vascular network could be observed by immunohistochemistry, but the spatial distribution of vessels could not be evaluated. The spatial distribution of vessels could be showed by angiography and CT scans. However, some of micro vessels were showed unclearly by CT scans. The number of blood vessels detected by immunohistochemistry was （26.50±3.02） vessels, significantly higher than those detected by angiography and CT scans that were （14.12±1.47） and （9.00±1.79） vessels, respectively. Combination of immunohistochemistry and angiography can evaluate the microvascular network at microscopic and macroscopic levels, which can provide the whole information of the vascular network.