MicroRNA-429对人肺癌A549细胞生长的影响

Effect of MicroRNA-429 on Cell Growth in Human Lung Cancer Cell Line A549

目的:探讨microRNA-429(miR-429)对人肺癌A549细胞增殖和凋亡的影响。方法:定量逆转录聚合酶链反应(qPCR)技术检测A549细胞瞬时转染miR-429mimic或inhibitor后miR-429的表达;通过MTT法和流式细胞术检测转染后细胞增殖能力和凋亡变化。结果:瞬时转染miR-429mimic 24h后,qPCR结果显示miR-429表达显著升高(9.3±0.3比1.0±0.1,P<0.05),而转染miR-429inhibitor可以显著降低miR-429表达(0.3±0.0比1.0±0.1,P<0.05);与对照组相比,过表达miR-429抑制肿瘤细胞增殖(2.8±0.2比5.0±0.3,P<0.05)并诱导凋亡(18.9±1.2比12.3±1.0,P<0.05),而下调miR-429表达促进肿瘤细胞增殖(6.3±0.4比5.0±0.3,P<0.05)并抑制凋亡(7.1±0.6比12.3±1.0,P<0.05)。结论:miR-429在肺癌中可能作为肿瘤抑制因子发挥功能。

Objective： To investigate the effect oI microRNA-429 （mlN-4Zg） on the proliferation and ap optosis of lung cancer cells. Methods： We detected expression of miR-429 after miR-429 mimic and its inhibitor were transiently transfected into human lung cancer cell line A549. The cell proliferation and apoptosis after transfection were analyzed by MTT and flow cytometry. Results： 24 h after miR-429 mimic transfection, the expression of miR-429 was significantly up-regulated （9.3±0.30 vs 1.0±0.08, P〈0.05）. However, miR-429 inhibitor transfection led to downregulation of miR-429（0.3±0.05 vs 1.0±0.08, P〈0.05）. Compared with control transfection, miR-429 overexpression inhibited tumor cell proliferation （2.8±0.21 vs 5.04±0.27, P〈0.05） and induced cell apoptosis significantly （18.9±1.2 vs 12.3±1.0, P〈0.05）. On the contrary, downregulation of miR-429 promoted tumor cell proliferation （6.3 ± 0.35 vs 5.0 ±0.27, P〈 0.05） and inhibited cell apoptosis significantly （7.1±0.6 vs 12.3±1.0, P〈0.05）. Conclusion： MiR-429 may serve as a tumor suppressor and may be a potential therapeutic target in lung cancer.