罗格列酮对硝酸甘油诱导偏头痛大鼠的保护作用

Protective Effect of Rosiglitazone on Rats with Migraine Induced by Nitroglycerin

目的观察罗格列酮对硝酸甘油诱导大鼠偏头痛模型的保护作用。方法 48只雄性SD大鼠随机分为模型组、罗格列酮组和对照组。模型组(按Tassorelli法皮下注射硝酸甘油复制大鼠偏头痛模型),罗格列酮组(于造模后30 min腹腔注射罗格列酮),对照组(不造模,皮下注射生理盐水)。观察大鼠行为学变化,采用免疫组化和Western blot法观察三叉神经颈复合体过氧化物酶体增殖物激活受体γ(PPARγ)及白介素-6(IL-6)、细胞间黏附分子-1(ICAM-1)、基质金属蛋白酶-9(MMP-9)表达。结果对照组三叉神经颈复合体中PPARγ为弱阳性表达,模型组为阳性表达,罗格列酮组为强阳性表达。模型组IL-6、ICAM-1和MMP-9表达高于对照组(P<0.05)。罗格列酮组大鼠耳发红、肢频繁挠头、爬笼次数增多、烦躁不安等症状减轻,IL-6、ICAM-1、MMP-9表达较模型组明显下降(P<0.05)。结论偏头痛时PPARγ表达增强;罗格列酮可通过活化PPARγ,下调IL-6、ICAM-1、MMP-9对偏头痛具有一定的保护作用。

Aim To observe the influence of rosiglitazone on migraine.Methods 48 male Sprague Dawley rats were divided into a model group,a rosiglitazone group and a normal control group.According to Tassorelli method the model group was made by copying rat migraine model.The rosiglitazone group was administrated after modeling of 30 min.After the success of modeling the changes of behavior and symptoms of rats were observed.The expression of peroxisome proliferator-activated receptor gamma（PPARγ） and interleukin-6 （IL-6）,intercellular adhesion molecule-1 （ICAM-1）,matrix metallopeptidase 9（MMP-9） in the trigeminocervical complex were detected by immunohistochemistry and Western blot.Results The expression of PPARγ in the model group was significantly higher than those in the normal control group.At the same time the expression of PPARγ in rats of the rosiglitazone group was significantly higher than those in the model group,while the expressions of IL-6,ICAM-1,MMP-9 in rats of model group were higher than those in the normal control group（P〈0.05）.In rosiglitazone group rats＇,symptoms such as ear redness,often scratching,climbing cage number increased,restlessness reduced and the expressions of IL-6,ICAM-1,MMP-9 decreased significantly （P〈0.05）.Conclusion During a migraine attack,the expression of PPARγ decrease.Rosiglitazone may have protective effect on migraine by down-regulating IL-6,ICAM-1 and MMP-9 via activating PPARγ,