摘要
目的观察CD3+T淋巴细胞在膀胱癌肿瘤上皮及癌旁正常尿路上皮中的分布差异,并探讨其意义.方法收集膀胱癌手术病例28例,术后取其膀胱癌组织标本及癌旁正常组织标本,采用免疫组织化学方法观察肿瘤上皮及癌旁正常尿路上皮中CD3+T淋巴细胞的数量及分布,计数2种上皮内的T淋巴细胞数量并比较其差异.结果在癌旁正常尿路上皮内可见较多的T淋巴细胞分布;而在肿瘤上皮中则少见T淋巴细胞分布,癌旁正常尿路上皮组每5个典型200倍视野中的上皮内T淋巴细胞平均数为(15±4.5)个,肿瘤上皮组则为(4±2.2)个,差异有统计学意义(P<0.01).结论膀胱癌肿瘤上皮与癌旁正常尿路上皮中CD3+T淋巴的分布存在着差异,这一现象可能与膀胱癌的免疫逃避机制及预后相关,值得进一步的深入研究.
Objective The purpose of this study was to observe the distribution of CD3＋T lymphocyte in the tissue of bladder cancer and epithelium of paracancer area, and analyze the significance. Methods Biopsy was performed in 28 patients with bladder cancer, and the distribution and number of CD3＋T lymphocyte in tissue of bladder cancer and epithelium of paracancer area were observed and compared using immunohistochemistry. Results Many of CD3＋T lymphocytes could be observed in the epithelium of paracancer tissues, but CD3＋T lymphocytes in cancer nests was few. The average number of CD3＋T lymphocytes in every 5 typical microscope visual fields of paracancer tissues and cancer nests was 15 ±4.5 and 4 ±2.2, respectively, and the difference was significant （P 〈0.05） . Conclusion Distribution of CD3＋T lymphocytes in bladder cancer nests and paracancer tissues was different, which may be related to the immune escape and prognosis of bladder cancer. It is worthy of further research.
出处
《昆明医科大学学报》
CAS
2014年第1期28-31,共4页
Journal of Kunming Medical University
基金
云南省卫生厅科技处内设机构基金资助项目(2011WS0082)
