GM-CSF及其受体与早期自然流产的关系研究

Study of Relationship of GM-CSF and Its Receptor with Early Spontaneous Abortion

目的探讨母胎界面局部细胞因子GM-CSF及其受体与早期自然流产的发生的关系.方法采集2009年8月至2011年12月间来云南省第三人民医院进行人工流产及自然流产病例的绒毛及脱膜组织各30例,用放射免疫法检测2组外周血中绒毛膜促性腺激素(HCG)含量,利用免疫组织化学和Western blot检测绒毛及脱膜组织中巨噬细胞集落刺激因子(GM-CSF)及其受体(GM-CSFR)表达情况.结果自然流产组HCG值与人工流产组相比明显下降(P<0.05).绒毛及脱膜组织中GM-CSF和GM-CSFR 2组均有表达.与人工流产组相比,绒毛组织中,自然流产组的GM-CSFR增加(P<0.05),GM-CSF的表达上调,但无统计学意义(P>0.05).脱膜组织中,自然流产组的GM-CSF和GM-CSFR均增加(P<0.05).结论在脱膜组织中,适量的GM-CSF和GM-CSFR浓度维持妊娠的继续,浓度过高则不利于妊娠,可能是自然流产的原因之一.

Objective To investigate the relationship of Maternal-fetal interface local cytokine GM-CSF and its receptor with the occurrence of early spontaneous abortion. Methods From August 2009 to December 2011,we collected 30 villi tissue samples with artificial abortion and 30 villi tissue samples with spontaneous abortion. At the same time, we collected 30 villi tissue samples with artificial abortion and 30 decidua tissue samples with spontaneous abortion,and 30 decidua tissue samples with spontaneous abortion. The human chorionic gonadotropin （ HCG） was detected by radioimmunoassay in every group. The expressions of granulocyte-macrophage colony-stimulating factor （GM-CSF） and granulocyte-macrophage colony-stimulating factor receptor （GM-CSFR ） were detected by Immunohistochemical staining and Western Blot in the villi tissue and decidua tissue in every group. Results The concentration of HCG in the spontaneous abortion group was lower than that in the artificial abortion group （ P〈0.05） . The protein expressions of GM-CSF and GM-CSFR were found in villus and deciduas tissues in both groups. The protein expression levels of GM-CSFR in the villus tissues were higher in spontaneous abortion group than those in artificial abortion group （ P〈0.05）, the protein expression of GM-CSF was upregulated, but there was no statistically significant difference between two groups. In deciduas tissues, the protein expressions of GM-CSF and GM-CSFR were upregualted in spontaneous abortion group （ P〈0.05） . Conclusions The suitable concentrations of GM-CSF and GM-CSFR in decidua tissue maintain the pregnancy continued. However,the higher concentrations of GM-CSF and GM-CSFR in the decidua tissue may be one of＆amp;nbsp;reasons of spontaneous abortion.