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miR-204对癫神经元中BDNF/TrkB信号通路的影响 被引量：6

The Study of miR-204 Regulates BDNF/TrkB Expression in Epileptic Neurons
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摘要 目的研究海马神经元癫模型中转染miR-204后对脑源性神经营养因子(BDNF)与TrkB通路调控作用的影响。方法取原代培养7d后的细胞,分为正常组、正常+BDNF组、癫组、癫+BDNF组、正常转染miR-204组、癫转染miR-204组、癫转染miR-204+BDNF组。癫组用无镁液处理3 h制作癫模型。制备成功miR-204慢病毒表达载体。采用免疫荧光、膜片钳及免疫印迹技术鉴定及观察miR-204对BDNF/TrkB通路表达的影响。结果 TrkB蛋白的磷酸化水平:正常+BDNF组高于正常组;癫+BDNF组低于正常+BDNF组,高于癫组;癫+miR-204+BDNF组高于癫+BDNF组和癫+miR-204组。结论 BDNF及miR-204可以改善BDNF/TrkB受抑制的状态,从而在癫疾病的缓解中可能发挥重要作用。 Objective To study the effect of miR-204 on BDNF/TrkB signaling and pathogenesis on the neuron model of epilepsy. Methods Primary hippocampal neurons were cultured in vitro for 7 days, and were divided into control group, control＋BDNF group, epilepsy group, epilepsy＋BDNF group , control＋miR204 group, epilepsy＋miR204 group and ep-ilepsy＋miR204＋BDNF group. The epilepsy model of hippocampal neurons was established by being exposed to Mg2＋free me-dia for 3 hours. The miR-204 lentivirus vector was constructed. The effect of miR-204 on BDNF/TrkB expression was detect-ed by immunohistochemistry, patch clamp and Western blot technique. Results Compared with the control group, the TrkB phosphorylation level was higher in control＋BDNF group. The TrkB phosphorylation level was lower in epilepsy＋BDNF group than that of control＋BDNF group, but it was higher than that of epilepsy group. The TrkB phosphorylation level was higher in epilepsy＋miR204＋BDNF group than that of epilepsy＋BDNF group and epilepsy＋ miR204 group. Conclusion BDNF and miR-204 can improve the inhibitory condition of BDNF/TrkB signaling and may play an important role in alleviat-ing epilepsy disease.
作者 常伟 潘立平 吴秋静 宋毅军
机构地区 天津医科大学 天津医科大学总医院神经内科
出处 《天津医药》 CAS 北大核心 2014年第3期214-216,I0005,共4页 Tianjin Medical Journal
基金 国家自然科学基金资助项目(项目编号:91132722、81071044)
关键词 癫痫 海马 神经元 脑源性神经营养因子 miR-204 miR-204 受体, trkB 微RNAs epilepsy hippocampus neurons receptor,trkB microRNAs brain-derived neurotrophic factor
分类号 R749.1 [医药卫生—神经病学与精神病学]
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同被引文献49

  • 1林卫红,孟红梅,吕玉丹,张淑琴.BDNF及其受体TrkB在实验性癫痫中的作用及意义探讨[J].中国神经免疫学和神经病学杂志,2007,14(1):11-13. 被引量：11
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天津医药
2014年 第3期

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