兔骨髓间充质干细胞在HA/CS支架中的增殖活性

Proliferation of MSCs from Rabbit BoneMarrow in HA/CS Support

目的:研究骨髓间充质干细胞(mesenchymal stem cells,MSCs)在羟基磷灰石(HA)/壳聚糖(CS)复合支架材料中的生长和增殖情况,探讨复合材料与细胞的相容性。方法:穿刺法抽取新西兰大耳白兔骨髓3ml,通过密度梯度离心法获取MSCs,体外贴壁培养,倒置显微镜下观察原代及传代细胞形态、数量生长情况,描绘生长曲线。将第2代细胞以高密度接种到支架材料中体外三维培养,观察其细胞相容性。结果:经密度梯度离心结合贴壁筛选得到纯化的间充质干细胞,细胞能够连续传至9代以上,第2、5代细胞增殖能力最强。细胞进行体外三维培养时,可在HA/CS复合支架材料上良好的附着、生长和增殖。结论:兔骨髓间充质干细胞能在体外成功培养,HA/CS复合支架与MSCs具有良好的细胞相容性。

Objective To investigate the growth and the proliferation of mesenchymal stem cells from rabbit bone marrow in the hydroxyl apatite （HA）/the shell polyose （CS） in the compound support material,discuss the compatibility between Compound materials and cell.Methods 3ml bone marrow was drawn by puncture from New Zealand white rabbits.Morrow mesenchymal cells were harvested through gradient centrifugation,then were cultivated.Primary cells and sub-cultured cells were estimated through observing their morphology and number,drawing growth curve.vaccinates in the support material the 2nd generation of cell by the high density in vitro three dimensional raise,observes his/her the cell compatibility.Results Pastes the wall after the density gradient offcenter to screen and unifies the transfer of generation to obtain the pure mesenchyma stem cell,the cell can pass on continuously to 9 generations above,2nd,5 generation of cell multiplication ability is strongest.When the cell carries on in vitro three dimensional raise,may on the HA/CS compound support material good adhere to stick cohere,the growth and the multiplication.Conclusion The rabbit marrow mesenchyma stem cell can in vitro success culture.The HA/CS compound support have the good cell compatibility for MSCs.