青蒿琥酯抑制增生性瘢痕成纤维细胞Smad3表达的研究

Artesunate inhibits expression of Smad3 in human hypertrophic scar fibroblasts

目的:研究青蒿琥酯(ART)抑制增生性瘢痕成纤维细胞的果蝇抗皮肤生长因子原体3(Smad3)的表达。方法:原代培养人皮肤增生性瘢痕成纤维细胞,以浓度为0,75,150,300,600μmol/L ART分别干预24h。荧光定量RT-PCR法检测其Smad3 mRNA的表达,Western blot法检测其Smad3蛋白表达。结果:各浓度ART作用于成纤维细胞后,荧光定量RT-PCR法检测发现Smad3 mRNA表达下调,与空白对照组比较,差异有统计学差异(P<0.01)。Western blot法检测发现各干预组Smad3蛋白表达下调,与空白对照组比较,差异均有统计学差异(P<0.01)。结论:ART能下调人皮肤增生性瘢痕成纤维细胞Smad3的mRNA及蛋白水平,通过抑制Smad3的表达从而减少胶原合成可能是ART抑制增生性瘢痕的机制之一。

Objective To explore the effect of artesunate （ART） on Mothers against DPP homolog 3 （Smad3） in hypertrophic scar derived fibroblasts and to elucidate its actual mechanism further.Methods Fibroblasts isolated from tissue specimens of hypertrophic scar were treated with for 24h.RT-qPCR method and Western Blot assessment were used to detect the mRNA and protein expression of Smad3.Results After incubation of fibroblasts with ART for 24h,RT-qPCR showed that the expression of Smad3 mRNA was decreased by various concentrations of ART,compared with the blank control group showed statistical differences （P＜0.01）.Western blot showed that the expression of Smad3 protein in fibroblasts significantly decreased by ART with a concentration dependent manner （P＜0.01）.Conclusion These results suggest that ART can effectively cause a decrease of Smad3 in mRNA and protein level,providing theoretical support for the application of ART to control hypertrophic scar.