p53基因敲除PLAG1转基因小鼠模型构建的研究

The construction of p53 gene knockout of plag1 transgenic mice with pleomorphic adenoma model

目的:建立p53基因敲除plag1转基因小鼠模型。方法:依托plag1转基因多形性腺瘤小鼠。取两只plag1+母鼠与一只p53+/-雄鼠杂交,分别取F1代中基因型为plag1+p53+/-的雌雄小鼠交配,得到基因型分别为plag1+p53-/-,plag1+p53+/-及plag1+p53+/+的三种多形性腺瘤小鼠,分别测量比较三种基因型多形性腺瘤的生长速度。结果:plag1+p53-/-基因型小鼠生长迟缓,寿命短,未能长出肿瘤便已死去。plag1+p53+/-肿瘤生长速度较同龄plag1+p53+/+小鼠相比较快,且有明显的差异(P<0.05)。结论:构建了p53基因敲除plag1转基因小鼠模型,且结果显示p53基因与PLAG1基因的相互作用和多形性腺瘤的生长速度增快有关。

Objective：To construct the plagl transgenic mice with p53 gene knockout model. Method：Two plagl＋ females were mated with one p53 heterozygote. All animals in this study were derived from these original founders so that both genotypes had the same mixed genetic background .F1 bred to generate plagl ＋p53-/-, plag＋p53＋/- and plagl ＋ p53＋ / - genotypes. Measuring the size of the tumors within the mice of the same age for every two days. Comparing and Analysing the differences between the pleomorphic adenoma of those three different genotypes. Result： The mice with plagl＋ p53-/- genotype died early even before the tumors started to grow because of their gene defects. The pleomorphic adenoma with plag＋ pA3＋/- genotype grew faster than the ones with plag＋ p53＋/- genotypes with higher tendency of malignance. Conclusion： The p53 gene defection plays a role in the growth of pleomorphic adenoma of the plagl transgenic mice.