中频脉冲电流经皮刺激运动性疲劳士兵肝区对血清GSH-PX、SOD、T-AOC活性及MDA含量的影响

Influence of percutaneous stimulation of hepatic region with mid-frequency pulse current on the activity of serum GSH-PX,SOD,T-AOC and the content of malondialdehyde in exercise-induced fatigued soldiers

目的探讨中频脉冲电流经皮刺激肝区对运动性疲劳士兵血清谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性,总抗氧化能力(T-AOC)以及丙二醛(MDA)含量的影响。方法选择60名无训练史的健康男性新兵,随机分为对照组和刺激组,每组30名。两组均进行为期5周的强化训练(每周一至周六训练6d,周日休息1d)以建立运动性疲劳模型,刺激组于每天训练结束后立即给予经皮肝区中频脉冲电流(频率1024Hz,间动周期1s,时间20min,输出强度≤80mA)康复治疗,两组均于第1、3、5周的周日在清晨空腹状态下采集静脉血,测定两组血清GSHPX、SOD活性,T-AOC及MDA含量。结果两组第5周末血清GSH-PX、T-AOC明显低于第3周末、第1周末,且第3周末明显低于第1周末(P<0.05,P<0.01);第3周末、第5周末血清SOD活性明显低于第1周末(P<0.01),但第5周末与第3周末比较差异无统计学意义(P>0.05);第5周末血清MDA含量明显高于第3周末、第1周末,且第3周明显高于第1周末(P<0.01)。与对照组比较,第1、3、5周末刺激组血清GSH-PX、SOD活性及T-AOC均明显增高,MDA含量明显降低,差异有统计学意义(P<0.05,P<0.01)。结论中频脉冲电流经皮刺激运动性疲劳士兵肝区可提高肝脏抗氧化酶的活性,增强机体抗氧化系统的能力,促进机体自由基消除,具有延缓运动性疲劳发生、促进疲劳恢复的作用。

Objective To explore the influence ofpercutaneous stimulation of the hepatic region with mid-frequency pulsed current on the serum activity of glutathione peroxidase （GSH-PX）, superoxide dismutase （SOD） and total antioxidant capacity and content of malondialdehyde （MDA） in exercise-induced fatigued soldiers. Methods Sixty healthy male recruits without training history were randomly divided into control group and stimulation group （n=30）. Subiects in both groups received intensive training for 5 weeks （trained from Monday to Saturday, and rest on Sunday） to establish an exercise-induced fatigue model. The recruits in stimulation group received rehabilitation therapy of percutaneous stimulation of the hepatic region with mid- frequency pulse current （frequency was 1024Hz, dynamic cycle Is, stimulation time 20min, output intensity 〈 80mA） after the training immediately. In every Sunday morning of the 1st, 3rd and 5th week, venous blood samples were obtained from recruits of both groups for determination of the serum activity of GSH-PX, SOD and T-AOC and content of MDA. Results In both groups, the serum activity of GSH-PX and T-AOC on 5th weekend was lower than that of 1st and 3rd weekends, and the serum activity of GSH-PX and T-AOC on 3rd weekend was lower than that of 1st weekend （P〈0.05, P〈0.01）; the serum activity of SOD on the 3rd and 5th weekends was lower than that of 1st weekend （P〈0.01）, while the SOD activity on the 3rd and the 5th weekend was similar （P〉0.05）; the serum MDA content on 5th weekend was higher than that of 3rd and 1st weekends, and the content on 3rd weekendwas higher than that of 1st weekend （P〈0.01）. The activity of GSH-PX, SOD and T-AOC increased and the MDA content decreased on 1st, 3rd and Sth weekends in stimulation group when compared with control group （P〈0.05, P〈0.01）. Conclusions The percutaneous stimulation of the hepatic region by mid-frequency pulsed current in exercise-induced fatigued soldiers may improve the activity of antioxidant enzymes in the liver, enhance the function of antioxidant system, promote free radical scavenging, delay the occurrence of and promote the recovery from exercise-induced fatigue.