琥珀酸脱氢酶B和丙酮酸脱氢酶激酶1在鼻咽癌组织中的表达及其临床意义

Expressions of SDHB and PDK1 and their clinical significance in nasopharyngeal carcinoma

目的:探讨琥珀酸脱氢酶(SDH)B和丙酮酸脱氢酶激酶(PDK)1在鼻咽癌组织中的表达及其临床意义。方法:采用免疫组化法检测78例鼻咽癌组织及35例鼻咽黏膜慢性炎症组织中SDHB和PDK1的表达情况,运用x2检验分析SDHB和PDK1表达与鼻咽癌患者临床病理因素的关系,运用Kaplan-Meter法分析其与预后的关系。结果:SDHB和PDK1在鼻咽癌组织中阳性表达率分别为39.7%(31/78)和53.8%(42/78),而在鼻咽黏膜慢性炎症组织中为62.9%(22/35)和20.0%(7/35),SDHB和PDK1表达均与鼻咽癌复发、颈部淋巴结转移相关(P<0.05),与患者性别、年龄等无显著相关(P>0.05)。K-M生存曲线提示SDHB阳性表达和PDK1阴性表达者的生存率分别高于SDHB阴性表达和PDK1阳性表达者,多因素Cox回归分析显示,T分级、临床分期、复发是影响鼻咽癌患者预后的独立因素,SDHB和PDK1表达不能作为影响鼻咽癌预后的独立因素。结论:SDHB和PDK1表达在鼻咽癌的发展、淋巴结转移、复发中起着重要作用,并可能对预后有影响,但SDHB和PDK1表达不是影响预后的独立因素。

Objective： To investigate the expressions of SDHB and PDK1 and their correlation with theclinicopathological factors and prognosis in nasopharyngeal carcinoma （NPC）. Methods： Immunohistochemstry was employed to analyze the expressions of SDHB and PDK1 in 78 specimens of NPC and 35 specimens of chronic nasopharyngitis tissues （CNT）. Retsults： SDHB expression detected in 39.7% of the cancer tissues was significantly lower than those in 62.9% of nasopharyngitis tissues （χ^2=5.183, P〈0.05）. While PDK1 expression detected in 53.8% of the cancer tissues was significantly higher than those in 20.0% of nasopharyngitis tissues （χ^2=1 1.269, P〈0.05）. SDHB and PDK1 expressions were significantly associated with lymph node metastasis, tumor recurrence, cranial nerve palsy, however, there was no significant association between the expression and gender, age. Kaplan-Meter survival analysis showed that the survival rates of positive SDHB expression and negative PDK1 expression were higher than that of negative SDHB expression and positive PDK1 expression. Multivariate Cox regression analysis showed that the development of NPC, lymph node metastasis and recurrence were independent prognostic factors for NPC, but the expressions of SDHB and PDK1 were not an independent factor. Conclusion： The expressions of SDHB and PDK1 play an important role in the development of NPC, lymph node metastasis and recurrence. SDHB and PDK1 expressions may have an impact on prognosis, but it was not independent factor affecting the prognosis in patients with NPC.