Ecdysoneless在胃癌组织中的表达及其意义

Expression and significance of ecdysoneless in gastric cancer

目的:探讨ecdysoneless(ECD)在胃癌中的表达及其与肿瘤病理参数和预后的关系。方法:Real-time PCR法检测56例胃癌组织及其对应癌旁正常胃黏膜组织中的ECD mRNA表达水平;免疫组化法检测100例胃癌组织中ECD蛋白的表达情况,分析ECD蛋白表达与胃癌病理参数及预后之间的相关性。结果:PCR结果显示ECD mRNA在胃癌组织中较癌旁正常胃黏膜组织中表达增高。100例胃癌组织中54例(占54.0%)ECD蛋白阳性表达。胃癌组织中ECD表达与肿瘤部位、肿瘤大小、分化程度、组织学类型无显著相关性(P>0.05),而与Lauren分型、淋巴结转移、脉管侵犯、远处转移、TNM分期密切相关(P<0.05)。KaplanMeier生存分析显示,ECD表达阳性组的平均生存时间为(35.8±1.9)月,显著低于阴性组的(45.2±1.9)月(P<0.01);ECD表达阳性组的五年生存率为24.1%(13/54),低于阴性组的50.0%(23/46)(P<0.01)。多因素Cox回归分析显示,TNM分期、Lauren分型可以作为胃癌的独立预后因子(P<0.05),而ECD表达不能作为胃癌的一个独立预后因子(P=0.212)。结论:ECD在胃癌中表达增高,其表达水平与Lauren分型、淋巴结转移、脉管侵犯、远处转移、TNM分期密切相关,且其高表达与胃癌预后相关。

Objective： To probe the ecdysoneless （ECD） expression and its correlation with clinical variables,such as clinical pathologic factors and prognosis in gastric cancer. Methods： ECD mRNA expression level in gastric cancer samples and matched normal gastric tissue from 56 patients was detected using Real-time PCR method. Immunohistochemical method was adopted to deteced the protein expression level of ECD in gastric cancer tiusse which with 5 years follow up. Results： ECD expression was up-regulated in gastric cancer tiusse compared with matched normal gastric tissue. Positive ECD immunoreactivity was seen in 54.0% of gastric cancer specimens, which was correlated with Lauren typing, lymph node metastasis, vessel invasion, distant metastasis, and TNM stage （P〈0.05）. Compared with ECD negative patients, ECD positive patients had significantly shorter survival times （35.8 ± 1.9 month vs 45.2 ± 1.9 month, P〈0.01） and short survival rate （24.1% vs 50.0%, P 〈0.01）. Multiariable COX regression analysis indicated that Lauren typing and TNM stage could serve as a dependent prognsis factor of gastric cancer （P〈0.01）, except ECD expression （P=0.212）. Conclusion： ECD expression is increased in gastric cancer tissue. The ECD expression correlates with Lauren typing, lymph node metastasis, vessel invasion, distant metastasis, and TNM stage and poor survival rate in gastric cancer.