L-Periaxin蛋白与Ezrin蛋白相互作用初步分析

Analysis of the Interaction between L-Periaxin and Ezrin

Periaxin是施旺(Schwann)细胞中特异表达的支架蛋白之一,参与髓鞘成熟及稳定.Periaxin的基因缺失或突变导致脱髓鞘型腓骨肌萎缩症(CMT)4F亚型的发生.埃兹蛋白(ezrin)是一种膜骨架连接蛋白,其在维持细胞形态和运动方面具有重要作用,与肿瘤细胞转移密切相关.本文通过免疫共沉淀、双荧光共定位、YFP双分子荧光互补、GST-pull-down等实验方法,分析了L-periaxin蛋白与ezrin蛋白之间的相互作用.结果表明,L-periaxin蛋白与ezrin蛋白可在细胞质中共定位并可发生相互作用.

Periaxin was first identified as a protein in novel cytoskeleton-associated proteins in peripheral nerve two PDZ-domain proteins, L- and S-periaxin, which are myelinating Schwann cells through a screen of myelination. The periaxin gene （PRX） encodes targeted differently region in Schwann cells for maintenance of peripheral nerve myelin. Mutations in the periaxin gene were known to cause autosomal recessive demyelinating Charcot-Marie-Tooth （CMT4F） and Dejerine-Sottas disease. Ezrin is a member of the ERM （Ezrin-Radxin-Moesin） protein family discovered as a cytoskeleton link protein. Ezrin was shown to be involved in the controlling of cell morphology, adhesion and movement, which required for cell growth, proliferation and survival. We performed co-immunoprecipitation, co-locolization, fluorescence complementation experiments and GST-pull-down assay to identify the interaction of L- periaxin with ezrin. The results indicated that periaxin with ezrin co-localized in cytoplasm and interacted with each other.