柳珊瑚共生菌Bucillus methylotrophicus发酵液化学成分研究

Study on Chemical Constituents from Marine Gorgonian-associated Bacterium Bucillus methylotrophicus

【目的】研究柳珊瑚Anthogorgiacaerulea共生细菌Bacillusmethylotrophicus发酵液中的化学成分。【方法】采用多种柱色谱和高效液相色谱分离技术，对柳珊瑚A．caerulea共生菌B．methylotrophicus发酵液中化学成分进行研究，运用理化和波谱分析方法鉴定其化学结构。【结果】从共生细菌B．Methylotro phicus发酵液中分离了6个化合物，分别鉴定为环（脯-甘）二肽（1）、环（S-脯-R-亮）二肽（2）、胸苷（3）、尿苷（4）、原儿茶酸（5）、3-Furancarboxylic acid-5-（hydroxmenthy）-Furan（6）。【结论】化合物1～6均是首次从该种细菌中分离得到，丰富了柳珊瑚共生菌B．methylotrophicus的化学多样性。

[Objective]To investigate the chemical constituents of the fermentation liquid from marine gorgonian-associated bacterium Bucillus methylotrophicus. [Method]The compounds were isolated and purified by column chromatography on silica gel and their structures were identified by spectral analyses and comparison with literatures. [ResuitlSix compounds were obtained from the fermentation liquid of B. rnethylotrophicus and characterized as Cyolo-（Pro- Gly） （1） ,Cyolo-（S-pro-R-leu） （2）, Thymidine （3）, Uridine （4）, Protocatechuic acid （5）, 3- Furancarboxylic acid-5- （hydroxmenthy）-Furan （6）. [Conclusion]Compounds 1 - 6 were isolated from B. methylotrophicus for the first time,which enriches the chemical diversity of marinegorgonian-associated bacterium Bucillus rneth ylotrophicus .