大孔吸附树脂分离黄粉虫黄酮及其抗氧化活性研究

Macroporous Adsorption Resin for Purification of Total Flavonoids from Tenebrio Molitor and Their Antioxidant Activity in Vitro

目的:研究大孔吸附树脂分离黄粉虫黄酮的工艺条件及其抗氧化功能。方法:比较4种树脂对黄粉虫黄酮的静态吸附和解吸性能,筛选出XDA-1型大孔吸附树脂用于分离黄粉虫黄酮,并测定纯化后黄粉虫黄酮的抗氧化性。以芦丁为阳性对照。结果:最佳工艺条件是:上样液质量浓度1.0 mg/mL、吸附流速2 BV/h、上样液pH5、上样量2 BV、洗脱流速2 BV/h、乙醇体积分数60%、洗脱液体积3 BV。经分离后黄粉虫黄酮纯度由3.74%提高到16.34%,纯化倍数为4.37。黄粉虫黄酮对羟基自由基、超氧阴离子自由基均有较强的清除作用,抗脂质体过氧化优于芦丁。结论:采用XDA-1型大孔吸附树脂分离黄粉虫黄酮操作简单、安全、成本低,有较高应用价值。黄粉虫黄酮具有较高的抗氧化能力,是1种天然有效的抗氧化剂。

Objective： In the present study, we used macroporous adsorption resin to purify total flavonoids from the crude extract of skimmed Tenebrio molitor powder and tested the antioxidant activity in vitro of the final purified product. Methods： The characteristics of adsorption and desorption of Tenebrio molitor flavonoids on four kinds of macroporous resins were compared, and XDA-1 was the selected resin considering the highest adsorption capacity and higher desorp-tion ratio. The in vitro antioxidant activity of flavonoids from Tenebrio molitor was evaluated by determining their scav-enging capacity against hydroxyl radicals and superoxide anions and the inhibitory effect on lipid peroxidation, and the rutin as a positive control. Results： The optimal purification technology was as follows： sample concentration 1.00 mg/ mL, sample loading flow rate 2 BV/h, sample pH 5.0, sample loading amount of 2 BV, 3 BV of 60% ethanol as the eluent at a flow rate of 2 BV/h. After the purification, the purity of total flavonoids increased from 3.74% to 16.34%. The purified flavonoids from Tenebrio molitor revealed a strong ability to scavenge hydroxyl radicals and superoxide an-ions, and also presented significantly higher inhibited effect on lipid peroxidation than rutin. Conclusion： XDA-I macro-porous adsorption resin is safe, economical and easy to use and can therefore be promisingly applied to separate and purify total flavonoids from Tencbrio molitor, and the purified flavonoids have excellent antioxidant activity, and can be used as a powerful natural antioxidant.