摘要
目的观察白血病抑制因子(Leukemia inhibitory factor,LIF)能否诱导后肾间充质细胞(metanephric mesenchymal cells,MMCS)分化形成肾上皮细胞,并探讨其机制。方法取孕11.5天的小鼠胚胎,分离后肾间充质组织,去除输尿管芽,Pax-2染色鉴定。对鉴定后的肾间充质细胞进行体外培养,并在培养液中加入细胞因子成纤维细胞生长因子(FGF2)、转化生长因子(TGF-α)和LIF进行诱导,用免疫荧光染色和RT-PCR检测后肾间充质细胞分化形成肾上皮细胞过程中的形态学和基因表达变化。结果经细胞因子LIF处理,后肾间充质细胞由排列杂乱无序的细胞发育形成有连续基底膜的肾小球。RT-PCR检测发现上皮细胞标记因子(E-caderin、CollagenⅣ、podocalyxin)的表达逐渐增高。结论 LIF能诱导后肾间充质细胞分化形成肾上皮细胞,可为肾脏疾病的细胞治疗提供实验依据。
Objective To investigate the biological characteristic in mesenchymal-epithial conversion. Methods We isolated the metanephric mesenchyme cells from E11.5 embryo without Ureteric Bud and identified it with Pax-2 staining. Results The isolated metanephric mesenchyme cells developed from a disorganised array into small chains with continuous basement membranes in the presence of Leukemia inhibitory factor （LIF）.And RT-PCR results showed the expression of the marker protein of epithelial cell,such as E-cadherin,Collagen Ⅳ,and podocalyxin were also affected. Conclusion LIF induced mesenchymal-epithial conversion in mouse metanephros.
出处
《中南医学科学杂志》
CAS
2014年第1期31-35,共5页
Medical Science Journal of Central South China
基金
湖南省自然科学衡阳市联合基金(10JJ9016)资助
关键词
后肾间充质细胞
上皮细胞
白血病抑制因子
诱导
metanephric mesenchyme cells
epithelial cells
Leukemia inhibitory factor
induce
