槲皮素对肺动脉高压大鼠肺组织细胞凋亡的影响

Effects of quercetin to apoptosis of pulmonary tissue of pulmonary arterial hypertension in rats

目的:观察槲皮素对肺动脉高压肺组织细胞凋亡的影响。方法:将成年雄性SD大鼠30只,随机分为三组:正常对照组(对照组)、野百合碱(MCT)诱导的肺动脉高压组(MCT组)和槲皮素预防组(预防组),每组10只。MCT组及预防组给予野百碱造模,对照组仅以等量0.9%氯化钠液注射,预防组在造模同时每天给予槲皮素100mg/(kg.d)灌胃,21d后测肺动脉压力并计算右心肥大指数,肺组织切片用免疫组化法和免疫印迹法观察大鼠肺组织细胞凋亡(Bcl-2)情况。结果:MCT组大鼠肺动脉平均压力[MCT组(42.13±6.28)vs.对照组(14.57±1.59),P<0.05;MCT组(42.13±6.28)vs.预防组(20.32±3.85),P<0.05]、右心肥大指数[MCT组(0.593±0.100)vs.对照组(0.241士0.050),P<0.05;MCT组(0.593±0.100)vs.预防组(0.290±0.065),P<0.05]、肺组织细胞的Bcl-2蛋白相对表达量[MCT组(0.964±0.009)vs.对照组(0.684±0.014),P<0.05;MCT组(0.964士0.009)vs.预防组(0.849±0.009),P<0.05]显著高于对照组及预防组;同时预防组大鼠肺动脉压力[预防组(20.32士3.85)vs.对照组(14.57±1.59),P<0.05]、右心肥大指数[预防组(0.290±0.065)vs.对照组(0.241±0.050),P<0.05]、肺组织细胞的Bcl-2蛋白相对表达量[预防组(0.849±0.009)vs.对照组(0.684士0.014),P<0.05]高于对照组。结论:槲皮素具有减缓MCT诱导肺动脉高压大鼠肺组织细胞凋亡的作用。

Objective：To investigate the effects of quercetin to apoptosis of pulmonary tissue on monocrotaline （MCT） induced pulmonary arterial hypertension （PAH） in rats.Methods：Tirty male SD rats were randomly divided into three groups with ten rats each group：control group,MCT group and prevention group.The rats in MCT group and prevention group were given a single subcutaneously injection of MCT and control group were injected with normal saline.The prevention group rats were received quercetin 100 mg/（kg·d） by gavage at the same time of molding.The mean pulmonary arterial pressure （mPAP） and right ventrilcular hypertrophy index（RVHI） of each groups was measured after 21day.The expression of Bcl-2 in pulmonary arterial was observed by immunohistochemistry and Western Blotting to evaluate the apoptosis of the pulmonary tissue.Results：The mPAP [（42.13 ± 6.28） in MCT group vs.（14.57 ± 1.59） in control group,P ＜ 0.05 ; （42.13 ± 6.28） in MCT group vs.（20.32 ± 3.85） in prevention group,P ＜ 0.05] and RVHI [（0.593 ± 0.100） in MCT group vs.（0.241 ± 0.050） in control group,P ＜ 0.05 ; （0.593 ± 0.100） in MCT group vs.（0.290 ± 0.065） in prevention group,P ＜ 0.05] and expression of Bcl-2 [（0.964 ± 0.009） in MCT group vs.（0.684 ± 0.014） in control group,P ＜ 0.05 ; （0.964 ± 0.009） in MCT group vs.（0.849 ± 0.009） in prevention group,P ＜ 0.05] were increased significantly in MCT group than that in control group and prevention group; In prevention group,The mPAP [（20.32 ± 3.85） in prevention group vs.（14.57 ± 1.59） in control group,P ＜ 0.05] and RVHI [（0.290 ± 0.065） in prevention group vs.（0.241 ± 0.050） in control group,P ＜ 0.05] and expression of Bcl-2 [（0.849 ± 0.009） in prevention group vs.（0.684 ± 0.014） in control group,P ＜ 0.05]were significantly increased than that in the control group.Conclusion：Quercetin could retard the apoptosis of the MCT-induced of pulmonary tissue of PAH.