采用单标稳定同位素示踪技术评价儿童铁利用率

Evaluation of iron utilization in children using single stable isotopes tracer

目的 利用单标稳定同位素示踪技术对儿童铁的利用率进行评价.方法 于2010年选择北京市某小学57名儿童,年龄为10 ～ 12岁,其中男童30名,女童27名.受试对象经口摄人人工富集制备的57FeSO4制剂,2次/d,5ml/次,连续5d,摄入的57Fe总量为每人30 mg.分别于口服前1天和服用后第14天采集受试者全血5 ml,并收集代谢期内全部粪便样品,经前处理后以原子吸收分光光度计和多接受等离子质谱仪测定样品中铁含量和丰度值,并结合受试者血容量计算和分析儿童铁的全血利用率情况.结果第14天时男童和女童血容量分别为（3.19±0.41）和（3.15±0.29）ml,差异无统计学意义（t=0.79,P＞0.05）；57Fe摄人量分别为（27.46±0.25） mg和（27.29±0.15）mg,差异无统计学意义（t=1.13,P＞0.05）；血液中57Fe的量（57Fecirc）分别为（5.92 ±0.71）和（6.30 ±0.65）mg,差异有统计学意义（=2.29,P＜0.05）；铁全血利用率分别为（20.41±2.03）％和（22.04 ±0.80）％,差异有统计学意义（t=2.51,P＜0.05）.结论单标稳定同位素示踪技术可用于儿童铁吸收率的评价,其中女童的铁全血利用率高于男童.

Objective To evaluate the iron utilization in children using single stable isotopes tracer.Methods 57 children aged from 10 to 12 from a primary school of Beijing in 2010 were selected,30 of them were boys and 27 were girls.All the subjects were given 5 ml artificial enriched 57FeSO4 twice per day within 5 days,and the total amount of 57Fe was 30 mg.5 ml blood were taken at 1 day before and 14 days after test,and all the feces during the test were collected.The samples were detected by AAS and MC-ICP-MS after pre-treatment to determine the content and abundances of iron in samples,then the iron utilization in whole blood were calculated.Results The blood volume of male and female subjects 14 days after test were（3.19 ±0.41） and（3.15 ±0.29）ml respectively,and there was no significantly difference （t =1.13,P 〉0.05） between them; The amount of 57Fe intake by male and female subjects were （27.46 ± 0.25） and（27.29 ±0.15）mg（t =1.13,P 〉0.05） ; The amount of 57Fe in blood were （5.92 ±0.71） and （6.30 ± 0.65）mg respectively （t =2.29,P 〈 0.05） ; The iron utilization in whole blood at 14 days of male and female subjects were （20.41 ± 2.03） % and （22.04 ± 0.80） % respectively,male subjects were significantly lower than females （t =2.51,P 〈 0.05）.Conclusion Single stable isotopes tracer can be used in iron utilization evaluation in children,and the iron utilization in whole blood of female children is higher than males.