葛根素对去卵巢大鼠海马CA1区神经元Caspase-3和Bcl-2表达的影响

Effect of Puerarin on Caspase-3 and Bcl-2 Expression of Hippocampal CA1 Neurons in Ovariectomized Rats

目的观察葛根素对去卵巢大鼠海马CA1区神经元Caspase-3和Bcl-2表达的影响,并探讨其作用机制。方法将SD雌性大鼠随机分为假手术组、模型组、阳性药组及葛根素高、中、低剂量组,除假手术组外,其余组均行去卵巢手术造模,各给药组给予相应药物腹腔注射,假手术组、模型组给予等生理盐水腹腔注射。采用免疫组化和RT-PCR法,观察去卵巢大鼠海马CA1区神经元Caspase-3和Bcl-2的表达。结果葛根素高剂量组大鼠海马CA1区Caspase-3表达明显减少(P<0.05);葛根素高、中剂量组大鼠海马CA1区Bcl-2表达明显增加(P<0.05,P<0.01),葛根素高剂量组大鼠海马CA1区Bcl-2 mRNA水平明显增加(P<0.05)。结论葛根素能下调去卵巢大鼠海马CA1区神经元Caspase-3表达,上调Bcl-2表达,对神经元结构异变具有一定的保护作用。

Objective To study the action of puerarin on Caspase-3 and Bcl-2 expression of hippocampal CA1 neurons in ovariectomized rats, and explore its mechanism. Methods SD female rats were randomly assigned into sham operation group, model group, premarin group and puerarin groups （120, 60, 30 mg/kg）. The model group and sham operation group were injected NS intraperitoneally, other groups were treated with corresping drugs for 30 d. Immunohistochemistry and RT-PCR were used to determine Caspase-3 and Bcl-2 expression of hippocampal CA1 neurons. Results Caspase-3 expression of hippocampal CA1 neurons was significantly decreased in high-dose puerarin group （P 〈0.05）. Bcl-2 expression of hippocampal CA1 neurons was significantly increased in high- and medium-dose puerarin groups （P〈0.05, P〈0.01）. Bcl-2 mRNA level of hippocampal CA1 neurons in high-dose puerarin group was significantly increased （P〈0.05）. Conclusion Puerarin can decrease Caspase-3expression and increase Bcl-2 expression of hippocampal CA1 neurons in ovariectomized rats, and has protective effect on neuronal structure.