徐州地区宫颈癌患者人乳头瘤病毒基因型检测分析

Human Papillomavirus Types in Invasive Cervical Cancer in Xuzhou Region

目的:通过对宫颈癌患者人乳头瘤病毒(HPV)基因检测分析,了解徐州地区宫颈癌HPV感染情况及HPV感染与宫颈癌临床生物学行为之间的关系。方法:采用核酸分子杂交系统对142例宫颈癌患者行HPV分型检测,并统计分析。结果:①142例宫颈癌患者中,136例HPV阳性,HPV感染率95.78%。其中单一感染117例(86.03%),多重感染19例(13.97%),两者感染率差异有统计学意义(P<0.01)。②共检测出13种基因型:单一感染8种,由高到低依次为HPV16(66.18%)、18(6.62%)、31(5.88%)、58(2.21%)、45(2.21%)、33(1.47%)、6(0.74%)和CP8304(0.74%)。而HPV68(2.94%)、52(2.21%)、66(1.47%)、39(0.74%)、11(0.74%)仅出现在多重感染中。HPV16总感染率为77.94%,明显高于其他各型(P<0.01)。而HPV18总感染率7.35%与HPV31、58、33相比差异无统计学意义(P>0.05)。③HPV感染率在宫颈癌各临床分期、组织病理学分级间差异无统计学意义(P>0.05)。④HPV多重感染与宫颈癌临床分期间无确定关系,但随着病理分级的加重呈降低的趋势。结论:徐州地区宫颈癌患者以HPV单一感染为主,主要为HPV16,其次为HPV18、31、58;未发现多重感染增加宫颈癌的发生风险;HPV感染不影响宫颈癌的进展及肿瘤细胞的分化。

Objective：This study investigated regional distribution in the contribution made by different human papilloma （HPV） types to invasive cervical cancer （ICC）, and to analyze the relation of HPV and clinical biological behavior in cervical cancer in Xuzhou region. Methods： Using nucleic acid hybridization technique to estimate and statistic analysis the HPV prevalence through 142 women with ICC. Results： ①Among the 142 women with ICC, a total of 136 participants （95.78%） had HPV infection. 19 of the 136 women （13.97%） who developed ICC were multiple HPV types infection, and 117 of them （86.03%） demonstrated a single HPV infection（P＜0.01）. ②The 13 most common HPV types were identified by this study. The 8 HPV types in single infection were, in order of decreasing prevalence, HPV16（66.18%）, 18（6.62%）, 31（5.88%）, 58（2.21%）, 45（2.21%）, 33（1.47%）, 6（0.74%） and CP8304（0.74%）; other HPV types in mutiple infections were HPV 68（2.94%）, 52（2.21%）, 66（1.47%）, 39（0.74%）, 11（0.74%）. HPV16 （77.94%） were found more commonly than other HPV types and these differences were statistically significant（P＜0.01）. Total infection of HPV18 was 7.35%. These were no statistically significant between HPV18 and HPV31,58,33（P＞0.05）. ③There was not considerable variation in the rate of HPV infection between different cervical cancer clinical stage and histopathological grading（P＞0.05）. ④Multiple infections seemed to have no relation to risk of ICC, whereas, to be decreasing with increasing pathological grade. Conclusions： A majority of ICC was associated with single HPV infection and approximately HPV16 was the predominant type followed by HPV18,31 and 58 in Xuzhou regions. Also there was not an increased risk of ICC associated with an abnormal cytology at baseline high-risk HPV types （HR-HPV） mutiple infections. HPV infection did not appear to be associated with progress of ICC and differentiation of the tumor cells.