硫自养高氯酸盐还原菌培养与驯化

Enrichment and cultivation of sulfur autotrophic perchlorate-reducing consortium

考察了以污水处理厂的好氧污泥作为接种污泥,以S0作为电子供体培养驯化硫自养高氯酸盐还原菌的过程,并利用PCR-DGGE技术对不同阶段的污泥样品进行群落分析。结果表明,接种污泥在经过短暂的适应期后能具有较高的还原ClO-4的性能,随着驯化时间的延长,降解速率显著提高。培养驯化过程中微生物种群结构发生了演替变化,菌种Dechloromonas sp.CL、Quadrisphaera granulorum、Comamonadaceae bacterium 32-4、Acidovorax caeni、filamentous bacterium Plant1Iso8、Candidatus Nitrospira defluvii存在整个驯化培养阶段。随着培养驯化时间的延长,菌种Bacteroidetes bacterium S22-33、Herbaspirillum huttiense逐渐消失,出现新菌种Methyloversatilis universalis、beta proteobacterium HTCC379、beta proteobacterium CDB21和Clostridium bifermentans,并逐渐成为优势菌种。

Enrichment and cultivation of sulfur autotrophic perchlorate-reducing consortium were investiga- ted by using aerobic sludge from sewage plant and sulfur as seeding sludge and electron donor, respectively. The seeding sludge had higher perchlorate removal efficiency after a short adaptation and the removal efficiency gradu- ally increased during the enrichment and cultivation. ClO4- could completely transform to Cl-. The successional change of microbial community was also investigated by PCR-DGGE technique. Some strains existed during the whole enrichment and cultivation, such as Dechloromonas sp. CL, Comamonadaceae bacterium 32-4, Quadrispha- era granulorum,Acidovorax caeni,filamentous bacterium Plantl Iso8 and Candidatus Nitrospira defluvii. With the time increasing of enrichment and cultivation,Bacteroidetes bacterium S22-33 and Herbaspirillum huttiense disap- peared, and Methyloversatilis universalis , beta proteobacterium HTCC379, beta proteobacterium CDB21 and Clostrid- ium bifermentans appeared as new species,which gradually became the predominant strains.