低温硝基苯降解菌选育及在土壤修复中的应用

Screening nitrobenzene-degrading bacterium and its application for soil remediation under low temperature condition

从硝基苯污染地下水中筛选出一株以硝基苯为唯一碳源和氮源的低温、高效降解菌,命名为XJ菌;初步鉴定XJ菌为革兰氏阴性微小短杆细菌、恶臭假单胞菌属,降解硝基苯遵循部分还原降解途径;在10℃下培养96 h,XJ菌对硝基苯的降解去除率达到91.1%,其中约有67.1%的硝基氮素转变成了氨氮。在10℃下利用XJ菌修复硝基苯污染土壤,修复时间为96 h时,硝基苯去除率达到85.7%,XJ菌密度及微生物脱氢酶活性保持较高的水平。本研究可为低温下硝基苯污染土壤的生物修复提供参考。

A strain of nitrobenzene-degrading bacterium named strain XJ, consuming nitrobenzene as the sole carbon source and nitrogen source, was screened successfully from nitrobenzene-contaminated groundwater under low temperature condition. Strain XJ was identified initially as small gram negative short rod bacterium and belonged to Pseudomonas Putida and followed the partial reductive pathway to degrade nitrobenzene. Cultured for 96 h,the removal rate of nitrobenzene degraded by strain XJ could reach 91.1% under 10℃ condition and 67. 7% nitrogen of nitryl was transformed into ammonia. During the remediation of nitrobenzene-contaminated soil with strain XJ at 10℃ ,when the remediation time was 96 h,the removal rate of nitrobenzene in soil could reach 85. 7% while the density of strain XJ and microbial dehydrogenase activities maintained a high level. This research could provide reference for bioremediation of nitrobenzene-contaminated soil under low-temperature condition.