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Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum

Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum
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摘要 Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids (mainly triacylglycerides, or TAGs) of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts (LACS3, G3PDH2, G3PDH3, and G3PDH5) were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum. Phaeodactylum tricornutum is a diatom that is rich in lipids.Recently,it has received much attention as a feedstock for biodiesel production.Nitrogen defi ciency is widely known to increase the content of neutral lipids(mainly triacylglycerides,or TAGs)of microalgae,including P.tricornutum,but the mechanism is unclear.In this study,we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P.tricornutum.After being cultured under nitrogen-free conditions for 0,4,24,48,72,120,and 168 h,the TAG contents of P.tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR.The results show that the abundances of four target gene transcripts(LACS3,G3PDH2,G3PDH3,and G3PDH5)were positively correlated with TAG content,indicating that these genes may be involved in TAG synthesis in P.tricornutum.The fi ndings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content of P.tricornutum.
出处 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第2期397-402,共6页 中国海洋湖沼学报(英文版)
基金 Supported by the National Basic Research Program of China(973 Program)(No.2011CB200901) the National Key Technology Research and Development Program(No.2011BAD14B01) the Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province(No.BS2010SW037) the National Natural Science Foundation of China(No.31270408)
关键词 Phaeodactylum tricornutum TAG content transcript abundance 三角褐指藻 关键酶基因 脂质合成 转录本 甘油酯 缺氮 酯含量 协会
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