复合酶法提取龙眼核总黄酮的工艺研究

Extraction Technology for Total Flavonoids from Longan Seeds by Composite Enzyme Method

目的:优化复合酶法提取龙眼核总黄酮的工艺条件。方法:在单因素试验的基础上,根据响应面法的Box-Behnke试验设计原理,以总黄酮含量为评价指标,分别考察酶解时间、pH和酶用量对龙眼核总黄酮提取效果的影响,并建立了总黄酮含量与各因素的多元二次回归数学模型。结果:各因素对总黄酮得率的影响次序为酶用量>酶解时间>pH值。最佳提取工艺条件为60%乙醇为提取溶剂,料液比为1∶30,复合酶(纤维素酶∶果胶酶=1∶2)用量1.12%,pH=6.0,酶解时间2.18 h。实际测得龙眼核总黄酮含量为30.40 mg/g,与预测值相符度为95%。结论:酶法提取条件温和、污染小、效率高,优选的工艺稳定可行,可为工业生产提供有益的参考。

OBJECTIVE：To optimize the extraction conditions of total flavonoids from longan seeds by composite enzyme method.METHODS：On the basis of single-factor experiment and based on Box-Behnke design principle in response surface method with total flavonoids as index for evaluation,the effects of enzyme hydrolysis time,pH value and enzyme amount on the total flavonoids yield were explored and the multiple quadratic regression mathematical model was established.The effects of enzyme hydrolysis time,pH value and enzyme amount on the total flavonoids yield were explored.RESULTS：The factors ranked by descending order of their effects on total flavonoids yield were as follows：enzyme amount ＞ enzyme hydrolysis time ＞ pH value.The optimum extraction conditions were concluded as follows：ethanol concentration of 60％,solid/liquid ratio of 1∶ 30,composite enzyme （cellulose∶ pectinase =1 ∶ 2） amount of 1.12％,pH of 6.0 and enzymolysis time of 2.18 hrs.The measured value of the yield of the total flavonoids was 30.40 mg/g,and the measured value and the predicated value had conformity rate of 95％.CONCLUSION：Enzyme extraction method was characterized by mild extraction conditions,high performance yet little pollution.The optimized extraction process is stable and feasible and it provides beneficial references for industrial production.