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禽腺病毒Ⅰ型hexon蛋白部分基因的原核表达及纯化 被引量:2

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摘要 根据GenBank上的CELO病毒Hexon基因序列,设计并合成了一对特异性引物,对CELO株进行PCR扩增、与PGEX-4T-1原核表达载体连接,转化大肠杆菌DH5a感受态细胞,再经0.2 mmol/L的IPTG诱导表达。SDS-PAGE电泳分析表明,表达的融合蛋白分子量为46.64 kD,占全菌蛋白的34%。经不同浓度尿素纯化后,纯度达到85%。Western-blotting结果表明:纯化的hexon蛋白具有抗原性,能与感染FAV的阳性血清反应。
作者 文艳玲
出处 《中国畜牧兽医文摘》 2014年第1期35-36,共2页
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参考文献5

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