川滇高山栎体胚诱导关键影响因素研究

Factors influencing the induction of somatic embryogenesis in Quercus aquifolioides Rehd.et Wils

【目的】探讨影响川滇高山栎体胚诱导的关键因素,为建立川滇高山栎体胚再生植株体系奠定基础。【方法】以川滇高山栎未成熟合子胚为外植体,研究基本培养基、植物生长调节剂配比、采样时期、抗氧化剂以及培养条件（光培养或暗培养）对其体胚诱导的影响。【结果】川滇高山栎未成熟合子胚在MS、1/2MS、B5和WPM基本培养基上均有体胚发生,其中MS基本培养基诱导效果较好,诱导率达60.0%,显著高于其他培养基,且愈伤组织量最多;以MS为基本培养基,同时添加1mg/L 6-BA和0.5mg/L 2,4-D,诱导率达83.3%,显著高于二者单独添加及其他组合处理;光照强度1 000~2 000lx,每天光照12h的光培养比暗培养更有利于体胚的发生;在林芝地区最佳采样时期为08-10左右;培养基中添加1.0g/L PVP可有效减轻褐变的发生。【结论】初步建立了川滇高山栎体胚诱导的培养体系。

[Objective] This study investigated the factors that influence the induction of somatic em- bryogenesis in Quercus aquifolioides Rehd. et Wils. [Method] Using immature zygotic embryos of aquifo- lioides as explants, effects of basic medium, hormones, concentrations of antioxidants medium, and culturing conditions on the induction of somatic embryogenesis were investigated. [Result] Somatic embryos were in- duced in all four tested basic medium including MS, 1/2 MS, Bs, and WPM. MS medium had the best induc- tion rate of 60.0~ with the maximum amount of callus. High induction rate of 83.3~ was achieved in MS medium supplemented with 1 mg/L 6-BA and 0.5 mg/L 2,4-D,which was significantly higher than all oth- er treatments. Light intensity of 1 000--2 000 lx for 12 h per day was more conducive to the occurrence of somatic embryogenesis than dark culture. Optimal sampling period was around August 10th and medium supplemented with 1.0 g/L PVP effectively reduced browning. [Conclusion] A culture system with opti- mal culture conditions for induction of somatic embryogenesis of Quercus aquifolioides Rehd. et Wils was established.