摘要
【目的】探讨嫁接对菊花耐热性的影响机理及对菊花不同器官耐热性的影响。【方法】比较40℃高温胁迫下菊花嫁接苗和不同生育期扦插苗的叶片、根系和茎段中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的变化与差异。【结果】菊花嫁接苗根茎叶中3种保护酶活性均显著高于扦插苗。与扦插苗相比,嫁接苗叶片、根系和茎段中SOD、POD和CAT活性分别平均提高11.6%,378.0%,260.1%;125.5%,131.3%,203.1%;90.1%,98.0%,90.5%。嫁接苗叶片中以SOD活性最大,均值达375U/g;根系中以POD活性最大,均值达605.87U/g;茎段中3种保护酶活性差异较小,以POD活性略高。【结论】嫁接是提高菊花耐热能力的一项有效措施,嫁接苗根茎叶的耐热性差别显著。
[Objective] This study investigated the effects of grafting on heat resistance of different or- gans of chrysanthemum. [Method] The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in leaves,roots and stems of grafted and cutting seedlings of chrysanthemum were studied at temperature of 40 ℃. [Result] The SOD,POD,and CAT activities in leaves,roots and stems of grafted chrysanthemum seedlings were significantly higher than that of cutting ones. The average SOD,POD,and CAT activities in leaves,roots and stems increased by 11.6% ,378.0% and 260.1% ;125.5%,131.3% and 203.1% ;and 90.1% ,98.0% and 90.5%,respectively. In leaves of grafted seedlings,SOD activity had the largest value of 375 U/g. In roots of grafted seedlings,POD activity had the largest value of 605.87 U/g. In stems of grafted seedlings, POD activity was slightly higher than the other two. [Conclusion] Grafting is an effective cultivation method to improve the resistance of chrysanthemum to heat stress. The heat resist- ances of different organs are significantly different.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2014年第1期125-131,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
安徽师范大学科研培育基金资助项目(2012rcpy049)
安徽师范大学生命科学学院省级重点实验室开放基金项目(ACLS2013-10)
高校博士点基金项目(20100146110027)
国家"863"计划项目(2006AA10010)
关键词
菊花
嫁接
高温胁迫
保护酶活性
Chrysanthemum morifolium
grafting
heat stress
protective enzymes activities