獭兔和中系安哥拉兔不同组织脂肪酸组成分析

Comparison on fatty acid compositions in different tissues of Rex rabbit and Angora rabbit

【目的】比较獭兔和中系安哥拉兔背最长肌、皮下和肠系脂肪组织中脂肪酸的组成。【方法】采用氯仿-甲醇超声法提取獭兔和中系安哥拉兔背最长肌、皮下和肠系脂肪组织中的油脂,用碱法甲酯化法对油脂进行处理,利用气相色谱-质谱联用仪对脂肪酸进行定性和定量分析。【结果】獭兔和中系安哥拉兔的不饱和脂肪酸平均相对含量分别为59.98%和66.10%,均高于饱和脂肪酸,是脂肪酸的主体成分。在獭兔和中系安哥拉兔的背最长肌、皮下和肠系脂肪组织中,软脂酸(十六碳酸,C16:0)、油酸(9-十八碳烯酸,C18:1)和亚油酸(9,12-十八碳二烯酸,C18:2)都是脂肪酸的主要组成成分。獭兔相对含量最大的脂肪酸是软脂酸(平均相对含量为27.63%),而中系安哥拉兔则含油酸最多(平均相对含量为32.41%)。【结论】獭兔和中系安哥拉兔脂肪酸组成具有共性,中系安哥拉兔脂肪酸种类较獭兔丰富。

[Objective] This study aimed to compare fatty acid composition in longissimus dorsi,subcu- taneous adipose tissue,and intestinal fat tissue of Rex rabbit and Angora rabbit. [Method] Chloroform methanol hyperacoustic method was used for lipid separation, then alkaline methyl esterification method was used for further process,and gas chromatography and mass spectrometry technique was used to deter- mine the fatty acid composition in longissimus dorsi, subcutaneous adipose tissue, and intestinal fat tissue of studied rabbits. [Result] Unsaturated fatty acids accounted for the majority of the averaged fatty acids in both Rex rabbit （59.98%） and Angora rabbit （66. 10%）. Palmitic acid （C16 ： 0）, oleic acid （C18 ： 1） and linoleic acid （C18： 2） were the main components. Content of oleic acid was the largest in Rex rabbit （27. 63%） ,while content of palmitic acid （C16-0） was the largest in Angora rabbit （32.41%）.[Conclusion] Fatty acid composition of the two rabbits was in common, and the type of fatty acids of Angora rabbit was richer than that of Rex rabbit..