天山雪莲黄酮类成分大孔树脂纯化工艺优选

Optimization of Purification Technology for Total Flavonoids from Saussureae Involucratae Herba by Macroporous Resin

目的:优选天山雪莲总黄酮的大孔树脂纯化工艺。方法:以芦丁为指标成分,通过静态吸附-洗脱试验筛选大孔树脂型号,采用单因素试验考察上样量、上样流速、洗脱流速、洗脱剂用量对天山雪莲黄酮纯化工艺的影响。结果:X-5型大孔树脂纯化效果最好,最佳纯化工艺条件为上样液质量浓度50 g·L-1,上样量6 BV,上样流速3 BV·h-1,加水3 BV洗去水溶性杂质,加20%乙醇6 BV除去其他杂质,加40%乙醇5 BV洗脱,洗脱流速3 BV·h-1,收集40%乙醇洗脱液;树脂纯化前后提取物中芦丁质量分数由1.07%提高至12.14%。结论:X-5型树脂可用于富集天山雪莲中黄酮类成分。

Objective： To optimize purification technology of total flavonoids from Saussureae Involucratae Herba by macroporous resin. Method： Taking rutin as index ingredient,macroporous resin model was screened by static adsorption-elution test,single factor tests were adopted to investigate effects of sample amount,sample flow rate,elution rate and other factors on purification technology of total flavonoids from Saussureae Involucratae Herba. Result： X-5 macroporous resin showed the best separation efficiency by compared with other resins,its optimum purification technology were as follows：the concentration of sample solution 50 g·L^-1,sample volume of 6 BV,adsorption rate of 3 BV·h^-1,washed impurities with 3 BV of water,removed other purities with 6 BV of 20% ethanol,eluted with 5 BV of 40% ethanol,elution velocity of 3 BV·h^-1,collected 40% ethanol eluent; After purified, the content of rutin increased from 1.07% in extract to 12.14% in ethanol eluent. Conclusion： X-5 macroporous resin could purify total flavonoids from Saussureae Involucratae Herba.