纤维素酶协同超声波法提取复方半边莲注射液的工艺优选

Optimization of Extraction Technology of Compound Lobelia Injection by Synergistic Action of Cellulase and Ultrasonic Method

目的:优选纤维素酶协同超声波法提取复方半边莲注射液的工艺条件。方法:在超声波条件固定的情况下,采用正交试验考察酶用量、酶解pH、酶解时间及酶解温度对复方半边莲注射液中野黄芩苷提取率的影响。采用HPLC测定野黄芩苷含量。结果:最佳工艺条件为酶解温度40℃,酶解pH 6.0,酶用量1 g(6.7 mg·g-1),酶解时间30 min;野黄芩苷质量浓度4.68 g·L-1,明显高于煎煮法(1.34 g·L-1)、单一超声波法(3.84 g·L-1)和单一纤维素酶法(2.26 g·L-1)。结论:纤维素酶协同超声波法提取复方半边莲注射液具有效率高、生产成本低、生产周期短等优点。

Objective： To optimize extraction technology of compound lobelia injection by synergistic action of cellulase and ultrasonic method. Method： Under these established ultrasonic conditions,orthogonal test was adopted to investigate effects of enzyme dosage,enzymolysis time,pH and temperature on yield of scutellarin in compound lobelia injection.The content of scutellarin was determined by HPLC. Result： Optimum technology conditions were as follows：enzymolysis temperature at 40 ℃,pH of 6.0,cellulase dosage 1 g（6.7 mg·g-1）,enzymolysis time 30 min;Under these conditions,the concentration of scutellarin in compound lobelia injection was 4.68 g·L^-1,which was significantly more than 1.34 g·L^-1 through decocting technology contained in registration standards,3.84 g·L^-1 through single ultrasonic extraction and 2.26 g·L^-1 through single cellulose extraction. Conclusion： Compared with the original extraction method contained in registration standards,synergistic action of cellulase and ultrasonic method had advantages of high extraction efficiency,less production cost and shorter production duration,etc.