锦灯笼果实提取工艺优选及抗氧化活性考察

Optimization of Extraction Technology for Physalis Calyx Seu Fructus Fruit and Investigation of its Antioxidant Activity

目的:优选锦灯笼果实的提取工艺并考察提取物的体外抗氧化活性。方法:以总黄酮、总酚酸提取量为指标,通过单因素试验和正交试验考察乙醇体积分数、提取时间及料液比对锦灯笼果实醇提工艺的影响,考察提取物对ABTS自由基的清除能力。结果:最佳提取工艺为加20倍量85%乙醇提取2次,每次0.5 h;总黄酮和总酚酸平均提取量分别为0.618,1.152 mg·g-1,RSD依次为1.66%,1.19%。结论:结合药效学试验筛选的提取工艺更加科学合理,锦灯笼果实具有一定的体外抗氧化能力。

Objective： To optimize extraction process of Physalis Calyx Seu Fructus fruit and investigate antioxidant activity of its extract. Method： With extraction amounts of total flavonoids and total phenolic acids as index,orthogonal test and single factor tests were employed to optimize ethanol extraction process of Physalis Calyx Seu Fructus fruit by taking ethanol concentration,extraction time and solid-liquid ratio as factors,and antioxidant activity of extract was investigated by observing its ability of scavenging ABTS free radical. Result： Optimum extraction technology was as follows：extracted twice with 20 times the amount of 85% ethanol,0.5 hour per time;Average extraction amounts of total flavonoids and total phenolic acids were 0.618,1.152 mg·g^-1 with RSD of 1.66% and 1.19%,respectively. Conclusion： It was reasonable to optimize extraction technology by taking pharmacodynamics test as index,Physalis Calyx Seu Fructus fruit had a certain in vitro antioxidant activity.