ZTC1+1-Ⅱ型澄清剂处理黄瑞香叶水提液的工艺优选

Optimization of Clarification Process for Water Extract of Daphne giraldii Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent

目的:优选ZTC 1+1-Ⅱ型澄清剂处理黄瑞香叶水提液的工艺条件。方法:以祖师麻甲素保留率和固形物去除率为指标,通过单因素试验考察药液质量浓度、温度、搅拌速度、水浴保温时间、澄清剂组份加入顺序和加入量对澄清效果的影响。采用HPLC测定祖师麻甲素含量,流动相甲醇-0.5%乙酸(25∶75),检测波长327 nm。结果:最佳澄清工艺为药液生药质量浓度0.1 g·mL-1,温度80℃,加入B组份溶液后加入A组份溶液,加入量分别为6%,3%,搅拌速度100 r·min-1,水浴保温时间10 min;祖师麻甲素保留率74.29%,固形物去除率38.80%。结论:ZTC 1+1-Ⅱ型澄清剂处理黄瑞香叶提取液的澄清效果良好,优选的澄清工艺稳定可行。

Objective： To optimize clarification process of water extract of Daphne giraldii leaves by ZTC 1＋1-Ⅱ type clarifying agent. Method： With retention rate of daphnetin and solid removal rate as indexes,single factor tests were adopted to optimize clarification process by taking extract concentration,reaction temperature,stirring speed,bath soaking time,adding order and amount of clarifying agents as factors.HPLC was adopted to determine the content of daphnetin with detection wavelength at 327 nm and mobile phase of methanol-0.5% acetic acid（25：75）. Result： Optimal clarification process was as follows：concentrated water extract to 0.1 g·mL^-1,reaction temperature at 80 ℃,added 6% of B component,then added 3% of A component,stirring speed of 100 r·min^-1,bath soaking time for 10 minutes;Under these conditions,retention rate of daphnetin and solid removal rate were 74.29% and 38.80%,respectively. Conclusion： Clarification effect of ZTC 1＋1-Ⅱ type clarifying agent for water extract of D. giraldii leaves is effective,and this optimized process was stable and feasible.