王不留行总皂苷提取工艺优选

Optimization of Extraction Process for Total Saponins from Vaccariae Semen

目的:优选王不留行总皂苷的提取工艺。方法:以总皂苷质量分数和干粉得率为综合评价指标,采用正交试验考察料液比、乙醇体积分数、超声时间、超声功率对超声波提取工艺的影响,考察提取时间、乙醇体积分数、料液比和提取次数对乙醇回流提取工艺的影响。结果:最佳超声提取工艺为加15倍量70%乙醇在640 W功率下超声提取2次,每次40 min;干粉得率7.34%,总皂苷质量分数10.92%。最佳乙醇回流提取工艺为加14倍量60%乙醇在微沸状态下提取2次,每次2 h;干粉得率7.17%,总皂苷质量分数9.01%。结论:王不留行总皂苷超声波辅助法的提取效果优于乙醇回流法,且工艺稳定可靠。

Objective： To optimize extraction technology of total saponins from Vaccariae Semen. Method： With dry powder yield and the mass fraction of total saponins as comprehensive evaluation index,orthogonal tests were adopted to optimize ultrasonic and ethanol reflux extraction processes by taking extraction time,solid-liquid ratio and ultrasonic power,ethanol concentration and extraction times as factors. Result： Optimum ultrasonic extraction conditions were as follows：extracted twice with 15 times the amount of 70% ethanol under 640 W,20 minutes per time;Dry powder yield and the mass fraction of total saponins were 7.34% and 10.92%,respectively.Optimum ethanol reflux extraction technology was as following：extracted twice with 14 times the amount of 60% ethanol under micro-boiling state,2 h per time;Dry powder yield and the mass fraction of total saponins were 7.17% and 9.01%,respectively. Conclusion： Ultrasonic assisted extraction method was better than ethanol reflux extraction method for total saponins from Vaccariae Semen,which were stable and reliable.