一测多评法测定银杏叶提取物中总黄酮醇苷的含量

Content Determination of Total Falconoid Glycosides in Ginkgo Biloba Extract by QAMS

目的:建立银杏叶提取物中总黄酮醇苷含量测定的一测多评法。方法:采用药典含量测定操作方法,以槲皮素为内标,通过测定山柰素、异鼠李素与槲皮素在固定配比和正交设计的不同配比下的响应因子,计算相对校正因子,进而考察相关影响因素并确立相对校正因子;样品测定中以槲皮素含量按相对校正因子直接计算出山柰素和异鼠李素的含量,三者相加计算总黄酮醇苷的含量,从而建立起仅以槲皮素为对照品的一测多评法,并通过与外标法实测值比较验证。结果:相对校正因子分别为f山柰素/槲皮素=1.020,f异鼠李素/槲皮素=1.033,RSD分别为1.23%,1.03%,重复性良好;8份样品的一测多评法计算值与外标法实测值之间没有显著性差异。结论:实验测得的相对校正因子及建立的银杏叶提取物中总黄酮醇苷含量测定的"一测多评法"准确可靠,为实际工作中银杏叶提取物中总黄酮醇苷的常规化、多批次含量测定带来了方便,具有较好的实用价值。

Objective： To establish a quantitative method of quantitative analysis of multi-component with a single-marker （QAMS） for the content determination of flavonoid glycosides in Ginkgo biloba extract. Method： According to the operating method in the Pharmacopoeia, quercetin was used as the internal standard, the response factors of kaempferol, isorhamnetin and quercetin were respectively measured under a fixed ratio and different ratios of orthogonal design to calculate the relative correction factor, the relative correction factors were established after investigating the related influencing factors. In the content determination of the samples, the relative correction factor were used to calculate the content of kaempferol and isorhamnetin. The content of flavonoid glycosides was determined by kaempferol, isorhamnetin and quercetin. The results of the QAMS method were verified by comparing with the external standard method. Result： The relative correction factors of kaempferol and isorhamnetin were 1.020 and 1.033 respectively;RSD was 1.23%,1.03% respectively, demonstrating good reproducibility. Two methods did not show significant difference in results. Conclusion： The relative correction factors and the QAMS methods for the content determination of flavonoid glycosides in Ginkgo biloba extract were accurate and reliable. This method is suitable for routine, multi-batch content determination of flavonoid glycosides in Ginkgo biloba extract, bringing the convenience to the actual content determination, and has good practical value.