HPLC同时测定降脂灵片中8个指标性成分的含量

Determination of the Content of Eight Ingredients in Jiangzhiling Tables by HPLC

目的：建立以高效液相色谱法同时测定降脂灵片中橙黄决明素、芦荟大黄素、大黄酸、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚的含量测定方法。方法：采用Phenomsil-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%磷酸水溶液，梯度洗脱，流速1.0 mL·min^-1，检测波长284 nm，柱温30 ℃。结果：在本色谱条件下，橙黄决明素、芦荟大黄素、大黄酸、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚的进样量分别在0.10-0.50，0.066-0.33，0.037-0.19，0.019-0.093，0.028-0.14，0.24-1.2，0.11-0.55、0.17-0.87 μg与色谱峰面积呈良好的线性关系；加样回收率（n=6）均在97.0%-102.9%，RSD均〈2.0%。结论：该法快速、准确，重复性好，可以满足降脂灵片的含量测定要求。

Objective： To establish an HPLC method for determination of aurantioobtusin,aloe-emodin,rhein,chrysoobtusin,obtusin,emodin,chrysophanol,physcion in Jiangzhiling tables. Method： The separation was performed on a Phenomsil-C18 ODS（4.6 mm×250 mm,5 μm） column with the gradient elution of acetonitrile-water of 0.1% phosphoric acid at the flow rate of 1.0 mL·min^-1. The detection wavelength at 284 nm and the column temperature was set at 30 ℃. Result： Aurantioobtusin,aloe-emodin,rhein,chrysoobtusin,obtusin,emodin,chrysophanol,physcion had good linearity in the ranges of 0.10-0.50,0.066-0.33,0.037-0.19,0.019-0.093,0.028-0.14,0.24-1.2,0.11-0.55,0.17-0.87 μg.repectively. The average recoveries of matters were 97.0%-102.9%, with RSDs of the recoveries were less than 2.0%. Conclusion： The developed method is rapid,accurate with high repeatability and stability,which is helpful to meet the content determination requirements of Jiangzhiling tables.