DNA条形码技术鉴定一种植物样品的研究

Study on Identification of an Unknown Sample with DNA Barcoding

目的:对一种未知的破碎的植物样品进行品种鉴定。方法:综合采用性状鉴定、显微鉴定、DNA条形码鉴定等方法,DNA条形码鉴定经过DNA提取、PCR扩增、测序,对ITS序列数据经Blast比对法、特异位点法、系统发育法三种方法进行分析。结果:该未知样品为伞形科植物茴香Foeniculum vulgare Mill.去掉果皮的种子。结论:中药鉴定技术能够解决实际工作中的未知样品品种鉴定问题,DNA条形码技术为中药鉴定提供一种新的方法,在未知样品的鉴定中发挥重要的参考作用。

Objective： To identify an unknown broken plant sample. Method： In this work,the method included macroscopical identification, microscopic identification, DNA barcoding identification was used. Using the DNA barcoding identification technology, DNA extraction, PCR amplification and sequencing, ITS sequence data were analyzed by three methods about the Blast comparison method, specific point method, system development method. Result： The unknown sample is the seeds of family umbrelliferae plant Foeniculum vulgare Mill. Conclusion： Traditional Chinese medicine authentication technology can solve the species identification problem of unknown sample in the actual work. DNA barcoding technology provide a new method for TCM authentication and play an important role of the identification of unknown sample.