加味四物四藤汤不同提取方法对大鼠急性痛风性关节炎的作用研究

Effect of Different Extracts from Modified Siwu Siteng Decoction on Acute Gouty Arthritis in Rats

目的:研究加味四物四藤汤不同工艺提取物(TFT-I,TFT-II)对尿酸钠晶体诱导的大鼠急性痛风性关节炎的影响。方法:选用SD大鼠,分为空白组、模型组、双氯芬酸钠组(0.007 g·kg-1)、TFT-I低、高剂量组(1.24,2.48 g·kg-1)、TFT-Ⅱ低、高剂量组(1.48,2.96 g·kg-1),各组均连续灌胃给药7 d,于第5天用尿酸钠晶体法造模。观察造模24 h后大鼠步态;用纸带法测定不同时相大鼠踝关节肿胀度;用酶联免疫吸附法测定大鼠踝关节组织中炎性因子白介素-1β(IL-1β)和环氧化酶-2(COX-2)的水平,采用同分异构体原理测定大鼠关节组织中前列腺素-2(PGE2)的水平。结果:与正常组大鼠8 h关节肿胀(0.07±0.01)cm相比,模型组大鼠关节肿胀于8 h达到高峰(0.52±0.02)cm(P<0.05),踝关节组织中IL-1β,COX-2和PGE2水平均升高,分别为(4.79±0.34),(14.94±1.50)μg·L-1和(1.57±0.16)×10-2(A)(P<0.05)。与模型组相比,TFT-I组对急性痛风性关节炎模型大鼠步态改善有显著性差异(P<0.05);TFT-I显著降低模型大鼠踝关节肿胀程度及关节组织中IL-1β,COX-2和PGE2水平,分别为(3.57±0.40),(11.72±1.17)μg·L-1和(1.20±0.11)×10-2A(P<0.01);TFT-II对急性痛风性关节炎模型大鼠步态、降低踝关节肿胀度及上述炎性因子无显著影响。结论:TFT-I对尿酸钠晶体诱导的大鼠急性痛风性关节炎关节肿胀有显著的改善作用,其机制与抑制炎性因子IL-1β,COX-2和PGE2的水平有关;在治疗痛风性关节炎时,加味四物四藤汤中来自"黄芪和四物汤"的醇溶性成分可能是本方剂的有效部位。

Objective： To study the effects of the different extracts from modified Siwu Siteng decoction （TFT-I, TFT-Ⅱ） on acute gouty arthritis in rats induced by monosodium urate crystals. Method： SD rats were divided into normal group,model group, positive medicine diclofenac sodium group（0.007 g·kg^-1）,TFT-I low and high dose group （1.24, 2.48 g·kg^-1）, TFT-Ⅱlow and high dose group（1.48,2.96 g·kg^-1）. The treatment groups were given corresponding drugs for 7 days. At 5th day monosodium urate method was used to establish model. Gaits of rats were observed at 24 h after modeling. Ankle joints＇ swelling degree at different time points after modeling was measured by paper tape. Interleukin-1β （IL-1β） and cyclooxygenase-2 （COX-2） levels were monitored by ELISA. Prostaglandine-2 （PGE2） levels were monitored by the principle of isomer. Result： Compared with normal group （0.07±0.01）cm, in model group, ankle joint swelling reached peak（0.52±0.02）cm at 8 h （P〈0.05）, and IL-1β, COX-2 and PGE2 levels were increased （P〈0.05）. Compared with model group, TFT-I could significantly improve gaits of rats （P〈0.05）, reduced ankle joints＇ swelling and the IL-1β,COX-2 and PGE2 level of rats＇ joint tissue （P〈0.01）. TFT-Ⅱ had no significant effect on swell improvement and inflammation factors inhibition. Conclusion： TFT-I has remarkable efficacy on symptom improvement in acute gouty arthritis rat induced by monosodium urate crystals, which may be related to its inhibiting IL-1β, COX-2 and PGE2 levels.