期刊文献+

噻螨酮与人血清白蛋白的作用机制 被引量:1

Studies on the interaction mechanism for human serum albumin with hexythiazox
原文传递
导出
摘要 用分子对接方法及紫外-可见吸收光谱、同步荧光光谱、三维荧光光谱等实验手段研究了噻螨酮(HEX)与人血清白蛋白(HSA)的相互作用及对HSA构象的影响.预测结果表明,HEX能与HSA发生相互作用,且作用位点site II比site I的打分小约4.5.实验结果表明,HEX猝灭HSA的内源荧光且作用机制为静态猝灭;HEX使HSA周围的微环境发生变化,导致蛋白质的肽链结构改变;298和291 K时HEX与HSA相互作用的结合常数(KA)和结合位点数分别为7.35×103 mol/L、0.82和1.02×104 mol/L、0.86,证实HEX仅在site II存在作用位点;HEX与Trp214的结合距离为3.01 nm,作用力主要为氢键、范德华力和疏水作用力.这些研究所获得的多种信息有助于在分子水平上理解农药对人体造成的毒性及可能的生物累积性. The interaction of human serum albumin (HSA) with hexythiazox (HEX) and the alterations of protein secondary structure in the presence of HEX were investigated by molecular docking, UV-vis absorption spectrometry, synchronous fluorescence spectrometry and three-dimensional fluorescence spectroscopy. Forecasting molecular docking results revealed that HEX could bind on site I and site II where it was easier to combine in HSA. The experiment results indicated that HEX had a strong ability to quench the intrinsic fluorescence of HSA through static quenching procedure. At the same time, HEX altered HSA surrounding microenvironment and led to change in the peptide chain structure of the protein. Binding affinity (KA) and the amounts of binding sites (n) between HEX and HSA at 298 and 291 K were estimated as 7.35 x 10^3 tool/L, 0.82 and 1.02 x 10^4 mol/L, 0.86, respectively, which confirmed that HEX could only bind on site II. The binding power between HEX and HSA involved mainly hydrogen bonds, van der Waals and hydrophobic forces, and an apparent distance between the Trp214 and HEX was 3.01 nm. The various informations of these studies help to understand toxicity of pesticide to humans and their bioaccumulative potential at the molecular level.
出处 《中国科学:化学》 CAS CSCD 北大核心 2014年第3期373-380,共8页 SCIENTIA SINICA Chimica
基金 自然科学基金重点项目(50830303) 长江学者与创新团队发展计划(IRT0853) 陕西省科学技术研究发展计划项目(2012k08-12)
关键词 噻螨酮 人血清白蛋白 分子对接 光谱法 hexythiazox, human serum albumin, molecular docking, spectroscopic methods
  • 相关文献

参考文献3

二级参考文献65

  • 1杨曼曼,杨频,张立伟.Study on Interaction of Caffeic Acid Series Medicine and Albumin by Fluorescence Method[J].Chinese Science Bulletin,1994,39(9):734-739. 被引量:9
  • 2陈珏,金泰廙,李凭建.镉对人脐静脉内皮细胞的不良效应[J].环境与职业医学,2004,21(3):165-169. 被引量:6
  • 3朱铿,童沈阳.荧光黄与蛋白质相互作用的研究[J].高等学校化学学报,1996,17(4):539-542. 被引量:43
  • 4李凡,赵华庭,黄海波.肝癌肺癌患者的头发分析[J].生物医学工程学杂志,1996,13(1):51-53. 被引量:3
  • 5Banerjee A, Dasgupta N, De B. In vitro, study of antioxidant activity of Syzygium cumini fruit. Food Chem, 2005, 90: 727-733.
  • 6Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 2007, 39: 44-84.
  • 7Sakurai T, He G, Matsuzawa A, Yu GY, Maeda S, Hardiman G, Karin M. Hepatocyte necrosis induced by oxidative stress and IL-1α release mediate carcinogen-induced compensatory proliferation and liver tumorigenesis. Cancer Cell, 2008, 14: 156-165.
  • 8Cathcart MK. Regulation of superoxide anion production by NADPH oxidase in monocytes/macrophages: Contributions to atherosclerosis. Arterioscler Thromb Vasc Biol, 2004, 24: 23-28.
  • 9Andreadis AA, Hazen SL, Comhair SA, Erzurum SC. Oxidative and nitrosative events in asthma. Free Radic Biol Med, 2003, 35: 213-225.
  • 10Emerit J, Edeas M, Bricaire F. Neurodegenerative diseases and oxidative stress. Biomed Pharmacother, 2004, 58: 39-46.

共引文献51

同被引文献14

引证文献1

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部