蛹虫草发酵菌丝体培养条件研究

Study on Culture Conditions of Cordyceps militaris Fermentation Mycelium

[目的]对蛹虫草菌丝生长条件进行优化,为发酵工业中液体深层发酵生产蛹虫草的药用有效成分提供参考。[方法]通过单因素试验,改变碳源、氮源、接种菌龄、培养基起始pH、培养周期、培养温度、摇床速度、接种量和装液量来确定蛹虫草菌丝生长最佳条件。[结果]最佳发酵条件为:碳源为蔗糖,浓度为2%,氮源为蛋白胨,浓度为1%,接种菌龄为36 h,培养基起始pH值为6.5,培养周期为6d,培养温度为24℃,摇床速度为200 r/min,在250 ml锥形瓶中的装液量为150 ml;在此条件下,菌丝体干重最大。[结论]该方法获得了蛹虫草C-7发酵菌丝体的最佳培养条件,为蛹虫草的进一步开发利用提供依据。

[ Objective] To provide reference for the production of effective medicinal ingredient by Cordyceps militaris in liquid fermentation. [ Method] Through single factor test, the best culture conditions of Cordyceps militaris was determined by changing carbon source, nitrogen source, age of pure culture, initial pH of medium, culture time, temperature, stirring rate and filling liquid. [ Result ] The optimal culture conditions： the optimal carbon source was 2% sucrose and the optimal nitrogen source was 1% tryptone. The best fermentation conditions of flask were ： age of pure culture 36h, initial pH 6.5, fermentation 6 d, temperature 24℃, stirring rate 200 r/min, filling liquid 150 ml/250 ml. [ Conclusion ] The best culture conditions for Cordyceps militaris C -7 mycelium were obtained, which will provide basis for further development and utilization.