脂肪酶水解菜籽油脚料工艺条件的优化

Optimization of Process Conditions of Hydrolysis from Rapeseed Oil Sediment by Lipase

[目的]优化脂肪酶水解菜籽油脚料的工艺,提高菜籽油脚料水解率。[方法]以菜籽油脚料为原料,选用黑曲霉脂肪酶水解菜籽油脚料,通过单因素试验、Box-Benhnken中心组合设计和响应面法对该脂肪酶水解油脂的工艺条件进行优化分析。[结果]单因素试验得出,黑曲霉脂肪酶水解菜籽油脚料的最适酶添加量为200 U/ml,底物浓度75 mg/ml,酶解pH 7.0,酶解温度40℃,酶解时间45 min以及摇床转数150 r/min,此时菜籽油脚料水解率为16.4%。利用Box-Benhnken中心组合设计和响应面法确定了最优工艺条件是:酶添加量245 U/ml,底物浓度为75 mg/ml,酶解pH 7.0,酶解温度是41℃,优化后的菜籽油脚料水解率达(26.92±0.86)%。[结论]研究可为菜籽油脚料的进一步开发利用提供参考依据。

[Objective ] To optimize process conditions of hydrolysis from rapeseed oil sediment by lipase. [ Method3 With rapeseed oil sediment as raw material, through single-factor experiment, Box-Benhnken compound design and response surface method, the technique conditions were optimized. [ Result] The optimal condition arc enzyme concentration 200 U/ml, substrate concentration 75 mg/ml, reaction system＇ s pH 7.0, reaction temperature 40 ℃, reaction time 45 min and table of revolution 150 r/min, the hydrolysis of rapeseed oil sediment is 16.4%. On this base, the four major factors were optimized by using Box-Benhnken central composite -orthogona] design and response surface methodology. The optimum conditions were determined as follows factors ： enzyme concentration 245 U/ml, substrate concentration 75 mg/ml, pH 7.0, temperature 41℃, after optimization of rapeseed oil sediment to hydrolysis rate is （26.92 ± 0.86）%. [ Conclusion ] The study can provide reference basis for further development and utilization of rapeseed oil sediment.