南蛇藤乙醇提取物对大鼠骨创伤愈合的影响

Effects of Celastrus Orbiculatu Ethanol Extract on Bone Wound Healing in Rats

目的研究南蛇藤乙醇提取物对大鼠骨创伤模型愈合的影响,为临床用药提供理论和实验依据。方法 40只SD大鼠随机分为假手术组、模型组、阳性药组、南蛇藤乙醇提取物高剂量组和低剂量组。复制大鼠骨创伤模型,除假手术组外,各组大鼠每天1次给药,连续给药45 d。给药期间观察大鼠的一般状况、伤肢活动和伤口愈合情况。末次给药后2 h处死大鼠,采血分离血清,测定血清中钙、磷浓度和碱性磷酸酶水平。取股骨照骨痂X光片,分析骨痂骨密度,用折力仪测定骨痂抗折强度。结果骨创伤大鼠股骨骨痂密度、抗折力、血清钙磷含量、碱性磷酸酶水平均降低,而阳性药和高剂量南蛇藤乙醇提取物能增加血清钙、磷含量和碱性磷酸酶水平,提高骨创伤大鼠骨痂密度和抗折力(P<0.05或P<0.01)。结论南蛇藤乙醇提取物能有效促进大鼠骨创伤愈合。

OBJECTIVE To study the effects of Celastrus orbiculatu ethanol extract on bone wound healing in rats. METHODS Forty Sprague-Dawley rats were randomly divided into 5 groups： pseudo surgery group, model group, drug-positive group, Celastrus orbiculatu ethanol extract high-dose and low-dose groups with 8 rats in each group. After surgical trauma femur, water or drugs were administrated to rats according to dose dosage for 45 days. The blood serum calcium, phosphorus, alkaline phosphatase contents were examined. The femur callus was took an X-ray photograph. Femur callus bone mineral density was calculated by computer software. The femur callus flexural strength was determined. RESULTS The blood serum calcium, phosphorus, alkaline phosphatase contents, the femur callus bone mineral density and the femur callus flexural strength were increased in the Celastrus orbiculatu ethanol extract high dose group and positive-drug group, which compared with the model group was statistically significantly different（P〈0.05）. CONCLUSION Celastrus orbiculatu ethanol extract can effectively promote the rat bone wound healing.