摘要
本实验通过12 5Ⅰ标记的鸡GnRH与同源血清 (抗体 )反应 ,建立了鸡GnRH放射免疫分析法 (RIA) ,并用以检测体外无血清培养的鸡卵泡细胞 ,证明卵泡膜细胞 (TC)和颗粒细胞 (GC)培养液中均存在GnRH Ⅱ样肽物质。并发现TC细胞培养液中的GnRH Ⅱ样肽含量在 72h内随培养时间加长而增多 ,而GC细胞培养液中GnRH样肽含量变化不明显。实验结果表明鸡卵泡细胞能产生GnRH
GnRH Radioimmunoassay (RIA)was established for detecting production of GnRH by serum free cultured follicular cells.GnRH like peptide was found in the medium of cultured theca cells (TC) and granulosa cells (GC) from chicken.The substance in the medium of TC increased gradually within 72h.But there were no significantly changes in the medium of GC.Those results demonstrated that the follicular cells of chicken could produce GnRH Ⅱ like peptide.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2000年第6期493-496,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目! (39370 5 14)
关键词
GNRH-Ⅱ样肽
鸡卵泡细胞
无血清培养
GnRH Ⅱ like peptide
Follicular cells of chicken
Serum free culture
Radioimmuno assay
