摘要
目的探索最佳的小鼠睾丸支持细胞分离、培养及鉴定方案。研究神经生长因子(nerve growth factor,NGF)对小鼠睾丸支持细胞生理状态的影响。方法通过胶原酶、胰酶等处理分离一周龄小白鼠睾丸支持细胞,通过油红O染色对分离得到的细胞进行鉴定;用加入NGF的培养基和对照培养基分别体外培养小鼠睾丸支持细胞,探索NGF对小鼠睾丸支持细胞生长情况的影响。结果分离得到了较高纯度的小鼠睾丸支持细胞,培养48小时后NGF组的小鼠睾丸支持细胞生长情况明显优于对照组。结论 NGF对小鼠睾丸支持细胞生长有着重要的作用。
Objective ①To explore the best scheme of separation、cultivation and appraisal in the mouse testis ser toli cells.②To study the nerve growth factor effects on mouse testis sertoli cells physiological state.Methods By colla genase enzyme and pancreatic enzyme working on the mouse testis sertoli cells which one week age,identify the ob tained cells by separation use the oil red O stain,Culture medium with join nerve growth factor to train the mouse testis sertoli cells and compared with the control group,To explore the nerve growth factor effects on mouse testis sertoli cells.Results obtained the mouse testis sertoli cells with the high purity,the mouse testis sertoli cells growth situation of model NGF group was obviously better than the control group after 48 hours.Conclusion Nerve growth factor on the growth of mouse testis sertoli cells plays an important role.
出处
《中国实验诊断学》
2014年第3期374-376,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省卫生厅课题(2011Z039)
