Survivin在甲状腺癌中的表达及临床意义

Expression of Survivin in Thyroid Carcinoma and its Clinical Significance

目的研究Survivin在正常甲状腺组织和甲状腺癌中的表达及其临床意义。方法采用免疫组化SP法及实时定量PCR方法对12例正常甲状腺组织和35例手术切除的甲状腺癌组织进行Survivin的检测。结果 1.SP法:Survivin在正常甲状腺组织中、乳头状癌中、甲状腺滤泡癌中、髓样癌中,阳性表达率分别为16.7%、60%、69.2%、91.6%,随甲状腺癌的组织学恶性度增高,阳性表达率增高,差异均具有显著性(P<0.0 5)。2.TR-PCR法:Survivin在正常甲状腺组织中、乳头状癌中、甲状腺滤泡癌中、髓样癌中的相对表达值为32.148±9.513、59.154±10.086、67.25±8.478、86.898±6.944,与正常甲状腺组织相比,Survivin在各组织分型的甲状腺癌中表达显著提高(P<0.05)。结论 Survivin与甲状腺癌的组织学类型密切相关,有望成为甲状腺癌患者预后判断的重要指标之一。

Objective To study the expression of survivin in Thyroid Carcinoma and its Clinical Significance.Methods Normal thyroid tissues from twelve people and thyroid cancer tissues obtained from 35 inpatients were detect the expression of survivin by immunohistochemistry SP method and RT-PCR method.Results 1.SP method：The positive rates of normal thyroid tissues、papillary thyroid carcinoma、follicular thyroid carcinoma、medulla thyroid carcinoma were 16.7 ％ 、60 ％ 、69.2 ％、91.6 ％,the survivin positive rate increased correlated with the higher histological grade of thyroid carcinoma.There was significant difference between every two groups statistically（P＜0.05）.2.RT-PCR meth od：The relative contents of survivin in normal thyroid tissues、papillary thyroid carcinoma、follicular thyroid carcinoma、medulla thyroid carcinoma were 32.148±9.513、59.154±10.086、67.25±8.478、86.898±6.944,Compared with normal thyroid tissue,the relative contents of survivn mRNA thyroid cancer tissues was correlated with histological type of differentiation（P＜0.05）.Conclusion Survivin is closely related to the histologic types of thyroid cancer,detecting of survivn may become one of the important prognosis indexes of thyroid cancer patients.